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Precisely sized separation of multiple particles based on the dielectrophoresis gradient in the z-direction

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Abstract

We present a new 3D dielectrophoresis-field-flow fraction (DEP-FFF) concept to achieve precise separation of multiple particles by using AC DEP force gradient in the z-direction. The interlaced electrode array was placed at the upstream of the microchannel, which not only focused the particles into a single particle stream to be at the same starting position for further separation, but also increased the spacing between each particle by the retard effect to reduce particle–particle aggregation. An inclined electrode was also designed in back of the focusing component to continuously and precisely separate different sizes of microparticles. Different magnitudes of DEP force are induced at different positions in the z-direction of the DEP gate, which causes different penetration times and positions of particles along the inclined DEP gate. 2, 3, 4, and 6 μm polystyrene beads were precisely sized fractionation to be four particle streams based on their different threshold DEP velocities that were induced by the field gradient in the z-direction when a voltage of 6.5 Vp–p was applied at a flow rate of 0.6 μl/min. Finally, Candida albicans were also sized separated to be three populations for demonstrating the feasibility of this platform in biological applications. The results showed that a high resolution sized fractionation (only 25% size difference) of multiple particles can be achieved in this DEP-based microfluidic device by applying a single AC electrical signal.

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References

  • Adams JD, Kim U, Soh HT (2008) Multitarget magnetic activated cell sorter. PNAS 105:18165–18170

    Article  Google Scholar 

  • Ateya DA, Erickson JS, Howell PB, Hilliard LR, Golden JP, Ligler FS (2008) The good, the bad, and the tiny: a review of microflow cytometry. Anal Bioanal Chem 391:1485–1498

    Article  Google Scholar 

  • Baylon-Cardiel JL, Jesús-Pérez NM, Chávez-Santoscoy AV, Lapizco-Encinas BH (2010) Controlled microparticle manipulation employing low frequency alternating electric fields in an array of insulators. Lab Chip 10:3235–3242

    Article  Google Scholar 

  • Beech JP, Jönsson P, Tegenfeldt JO (2009) Tipping the balance of deterministic lateral displacement devices using dielectrophoresis. Lab Chip 9:2698–2706

    Article  Google Scholar 

  • Chang HC, Yeo LY (2010) Electrokinetically driven microfluidics and nanofluidics. Cambridge University Press, New York

    Google Scholar 

  • Chen D, Du H (2007) A dielectrophoretic barrier-based microsystem for separation of microparticles. Microfluid Nanofluid 3:603–610

    Article  Google Scholar 

  • Cheng IF, Chang HC, Hou D, Chang HC (2007) An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting. Biomicrofluidics 1:021503

    Article  Google Scholar 

  • Cheng IF, Froude VE, Zhu Y, Chang HC, Chang HC (2009) A continuous high-throughput bioparticle sorter based on 3D traveling-wave dielectrophoresis. Lab Chip 9:3193–3201

    Article  Google Scholar 

  • Cheng IF, Lin CC, Lin DY, Chang HC (2010a) A dielectrophoretic chip with a roughened metal surface for on-chip surface-enhanced Raman scattering analysis of bacteria. Biomicrofluidics 4:034104

    Article  Google Scholar 

  • Cheng IF, Senapati S, Cheng X, Basuray S, Chang HC, Chang HC (2010b) A rapid field-use assay for mismatch number and location of hybridized DNAs. Lab Chip 10:828–831

    Article  Google Scholar 

  • Cheng IF, Chung CC, Chang HC (2011) High-throughput electrokinetic bioparticle focusing based on a travelling-wave dielectrophoretic field. Microfluid Nanofluid 10:649–660

    Article  Google Scholar 

  • Cheung K, Gawad S, Renaud P (2005) Impedance spectroscopy flow cytometry: on-chip label-free cell differentiation. Cytom A 65A:124–132

    Article  Google Scholar 

  • Choi S, Song S, Choi C, Park JK (2009) Microfluidic Self-sorting of mammalian cells to achieve cell cycle synchrony by hydrophoresis. Anal Chem 81:1964–1968

    Article  Google Scholar 

  • Chung TD, Kim HC (2007) Recent advances in miniaturized microfluidic flow cytometry for clinical use. Electrophoresis 28:4511–4520

    Article  Google Scholar 

  • Durr M, Kentsch J, Muller T, Schnelle T, Stelzle M (2003) Microdevices for manipulation and accumulation of micro- and nanoparticles by dielectrophoresis. Electrophoresis 24:722–731

    Article  Google Scholar 

  • Han KH, Frazier AB (2008) Lateral-driven continuous dielectrophoretic microseparators for blood cells suspended in a highly conductive medium. Lab Chip 8:1079–1086

    Article  Google Scholar 

  • Han KH, Han SI, Frazier AB (2009) Lateral displacement as a function of particle size using a piecewise curved planar interdigitated electrode array. Lab Chip 9:2958–2964

    Article  Google Scholar 

  • Hu X, Bessette PH, Qian J, Meinhart CD, Daugherty PS, Soh HT (2005) Marker-specific sorting of rare cells using dielectrophoresis. PNAS 102:15757–15761

    Article  Google Scholar 

  • Huang Y, Wang XB, Becker FF, Gascoyne PRC (1997) Introducing dielectrophoresis as a new force field for field-flow fractionation. Biophys J 73:1118–1129

    Article  Google Scholar 

  • Ibrahim SF, van den Engh G (2003) High-speed cell sorting: fundamentals and recent advances. Curr Opin Biotechnol 14:5–12

    Article  Google Scholar 

  • Jones TB (1995) Electromechanics of particles. Cambridge University Press, New York

    Book  Google Scholar 

  • Khoshmanesh K, Zhang C, Tovar-Lopez FJ, Nahavandi S, Baratchi S, Mitchell A, Kalantar-zadeh K (2010) Dielectrophoretic-activated cell sorter based on curved microelectrodes. Microfluid Nanofluid 9:411–426

    Article  Google Scholar 

  • Khoshmanesh K, Nahavandi S, Baratchi S, Mitchell A, Kalantar-zadeh K (2011) Dielectrophoretic platforms for bio-microfluidic systems. Biosens Bioelectron 26:1800–1814

    Article  Google Scholar 

  • Li Y, Dalton C, Crabtree HJ, Nilsson G, Kaler KVIS (2007) Continuous dielectrophoretic cell separation microfluidic device. Lab Chip 7:239–248

    Article  Google Scholar 

  • Lillehoj PB, Tsutsui H, Valamehr B, Wu H, Ho CM (2010) Continuous sorting of heterogeneous-sized embryoid bodies. Lab Chip 10:1678–1682

    Article  Google Scholar 

  • Lin CH, Lee CY, Tsai CH, Fu LM (2009) Novel continuous particle sorting in microfluidic chip utilizing cascaded squeeze effect. Microfluid Nanofluid 7:499–508

    Article  Google Scholar 

  • Muller T, Gradl G, Howitz S, Shirley SG, Schnelle T, Fuhr G (1999) A 3-D microelectrode system for handling and caging single cells and particles. Biosens Bioelectron 14:247–256

    Article  Google Scholar 

  • Pethig R (2010) Review article—Dielectrophoresis: status of the theory, technology, and applications. Biomicrofluidics 4:022701

    Article  Google Scholar 

  • Pethig R, Talary MS, Lee RS (2003) Enhancing traveling-wave dielectrophoresis with signal superposition. IEEE Eng Med Biol Mag 22:43–50

    Article  Google Scholar 

  • Pohl HA (1978) Dielectrophoresis: the behavior of neutral matter in nonuniform electric fields. Cambridge University Press, New York

    Google Scholar 

  • Takahashi T, Ogata S, Nishizawa M, Matsue T (2003) A valveless switch for microparticle sorting with laminar flow streams and electrophoresis perpendicular to the direction of fluid stream. Electrochem Commun 5:175–177

    Article  Google Scholar 

  • Wang L, Flanagan LA, Jeon NL, Monuki E, Lee AP (2007) Dielectrophoresis switching with vertical sidewall electrodes for microfluidic flow cytometry. Lab Chip 7:1114–1120

    Article  Google Scholar 

  • Yang J, Huang Y, Wang XB, Becker FF, Gascoyne PRC (2000) Differential analysis of human leukocytes by dielectrophoretic field-flow-fractionation. Biophys J 78:2680–2689

    Article  Google Scholar 

  • Yu CH, Vykoukal J, Vykoukal DM, Schwartz JA, Shi L, Gascoyne PRC (2005) A three-dimensional dielectrophoretic particle focusing channel for microcytometry applications. J Microelectromech Syst 14:480–487

    Article  Google Scholar 

  • Zhu J, Tzeng TRJ, Hu G, Xuan X (2009) DC dielectrophoretic focusing of particles in a serpentine microchannel. Microfluid Nanofluid 7:751–756

    Article  Google Scholar 

Download references

Acknowledgments

The work was supported by the funds from Multidisciplinary Center of Excellence for Clinical Trial and Research (DOH100-TD-B-111-002), Medical Device Innovation Center, National Cheng Kung University, and the NSC under Grant (NSC 99-2628-B-006-001-MY3 and NSC 100-2221-E-006-026-MY3). We also thank National Nano Device Laboratory (NDL) and Southern Taiwan Nanotechnology Research Center for supplying microfabrication equipment.

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Correspondence to Hsien-Chang Chang.

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Shu-Hsien Liao and I-Fang Cheng contributed equally to this work.

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Liao, SH., Cheng, IF. & Chang, HC. Precisely sized separation of multiple particles based on the dielectrophoresis gradient in the z-direction. Microfluid Nanofluid 12, 201–211 (2012). https://doi.org/10.1007/s10404-011-0863-9

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  • DOI: https://doi.org/10.1007/s10404-011-0863-9

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