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Spectral Slope from the Endoscopically-Normal Mucosa Predicts Concurrent Colonic Neoplasia: A Pilot Ex-Vivo Clinical Study

  • Original Contribution
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Diseases of the Colon & Rectum

Abstract

Purpose

We previously reported that analysis of histologically normal intestinal epithelium for spectral slope, a marker for aberrations in nanoscale tissue architecture, had outstanding accuracy in identifying field carcinogenesis in preclinical colorectal cancer models. In this study, we assessed the translatability of spectral slope analysis to human colorectal cancer screening.

Methods

Subjects (n = 127) undergoing colonoscopy had spectral slope determined from two endoscopically normal midtransverse colonic biopsies using four-dimensional elastic light-scattering fingerprinting and correlated with clinical findings.

Results

Four-dimensional elastic light-scattering fingerprinting analysis showed the submicron particles size progressively shifted toward larger sizes in subjects harboring neoplasia. There was a corresponding decrease in spectral slope values from the endoscopically normal mucosa in subjects harboring adenomas (n = 41) and advanced adenomas (n = 10), compared to neoplasia-free subjects (P ≤ 0.00001). These factors did not appear to be confounded by either age or adenoma location. For detecting advanced adenomas, spectral slope had a negative and positive predictive value of 95 percent and 50 percent respectively.

Conclusions

We demonstrate, for the first time, that spectral slope in “normal” mucosa can accurately risk-stratify patients for colonic neoplasia. This proof of concept study serves to underscore the promise of four-dimensional elastic light-scattering fingerprinting analysis for colorectal cancer screening.

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Acknowledgments

The authors thank Drs. Eric Elton, Jonathan Horwitz, Mick Meiselman, Yolandra Johnson, Sonali Master, and Eric Cohen for their assistance in patient recruitment and Ms. Beth Parker for assistance.

Author information

Authors and Affiliations

  1. Department of Internal Medicine, Evanston Northwestern Healthcare Research Institute, 2650 Ridge Avenue, G208, Evanston, Illinois, 60201, USA

    Hemant K. Roy M.D., Michael J. Goldberg M.D., Randall E. Brand M.D., Nahla Hasabou M.D. & Mohammed Jameel M.D.

  2. Biomedical Engineering Department, Northwestern University, Evanston, Illinois, USA

    Vladimir Turzhitsky M.S., Young L. Kim Ph.D., Yang Liu Ph.D. & Vadim Backman Ph.D.

  3. Department of Surgery, Evanston-Northwestern Healthcare, Evanston, Illinois, USA

    Joseph P. Muldoon M.D.

  4. Department of Pathology, Evanston-Northwestern Healthcare, Evanston, Illinois, USA

    Curtis Hall M.D.

Authors
  1. Hemant K. Roy M.D.
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  2. Vladimir Turzhitsky M.S.
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  3. Young L. Kim Ph.D.
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  4. Michael J. Goldberg M.D.
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  5. Joseph P. Muldoon M.D.
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  6. Yang Liu Ph.D.
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  7. Randall E. Brand M.D.
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  8. Curtis Hall M.D.
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  9. Nahla Hasabou M.D.
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  10. Mohammed Jameel M.D.
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  11. Vadim Backman Ph.D.
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Corresponding author

Correspondence to Hemant K. Roy M.D..

Additional information

Drs. Roy, Goldberg, and Backman are cofounders and stock holders in American BioOptics.

This work was supported by research grants from the National Institutes of Health (U01CA111257, R01CA112315, R01CA128641, R01CA109861, R42CA130508 and R01EB003682), National Science Foundation (CBET-0733868), and Coulter Foundation.

Read meeting of The American Society of Colon and Rectal Surgeons, Seattle, Washington, June 3 to 7, 2006.

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Roy, H.K., Turzhitsky, V., Kim, Y.L. et al. Spectral Slope from the Endoscopically-Normal Mucosa Predicts Concurrent Colonic Neoplasia: A Pilot Ex-Vivo Clinical Study. Dis Colon Rectum 51, 1381–1386 (2008). https://doi.org/10.1007/s10350-008-9384-3

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  • DOI: https://doi.org/10.1007/s10350-008-9384-3

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