European Journal of Wildlife Research

, Volume 61, Issue 1, pp 135–141

Hematology and serum biochemistry values of free-ranging Iberian wolves (Canis lupus) trapped by leg-hold snares

  • Nuno Santos
  • Helena Rio Maior
  • Mónia Nakamura
  • Sara Roque
  • Ricardo Brandão
  • Francisco Petrucci-Fonseca
  • Vicente Palacios
  • Emílio Garcia
  • José Vicente López-Bao
  • Luis Llaneza
  • Francisco Álvares
Original Paper

DOI: 10.1007/s10344-014-0883-3

Cite this article as:
Santos, N., Maior, H.R., Nakamura, M. et al. Eur J Wildl Res (2015) 61: 135. doi:10.1007/s10344-014-0883-3
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Abstract

Hematology and serum biochemistry are important tools in assessing the health and physiological status of wildlife populations. Nevertheless, studies on free-ranging wolves (Canis lupus) are scarce, and no reference values are available neither for Iberian wolves nor for wolves captured with leg-hold snares. We report 37 hematology and serum biochemistry variables obtained from 26 free-ranging Iberian wolves captured with leg-hold snares between 2007 and 2014, including variables previously not reported in the literature. The values obtained are similar to the published reference intervals for Scandinavian wolves captured by darting from a helicopter, except for higher values for mean corpuscular hemoglobin concentration (MCHC), red blood cell distribution width (RDW), leukocyte count, creatinine kinase (CK), γ-globulins, and total bilirubin (TBIL) and lower values for alkaline phosphatase (ALP). We propose that differences in leukocyte count, CK, and TBIL are related to the method of capture, while differences in RDW, MCHC, ALP, and γ-globulins could reflect physiological adaptations to environmental conditions, sampling, or pre-analytical artifacts. Lymphocyte count was lower and neutrophil/lymphocyte ratio was significantly higher in older, reproductive females, while ALP and phosphorus were higher in juvenile wolves. For the first time, we describe hematology and serum biochemistry values of free-ranging Iberian wolves captured with leg-hold snares. The data reported here is the first published reference for wolves captured with similar methods and for monitoring Iberian wolves populations’ physiological and health status.

Keywords

Iberian wolf Hematology Serum biochemistry Reference intervals Leg-hold snares 

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Nuno Santos
    • 1
    • 2
  • Helena Rio Maior
    • 2
  • Mónia Nakamura
    • 2
  • Sara Roque
    • 3
    • 4
  • Ricardo Brandão
    • 5
  • Francisco Petrucci-Fonseca
    • 3
    • 4
  • Vicente Palacios
    • 6
  • Emílio Garcia
    • 6
  • José Vicente López-Bao
    • 7
    • 8
  • Luis Llaneza
    • 2
    • 6
  • Francisco Álvares
    • 2
  1. 1.ICVS—Instituto de Investigação em Ciências da Vida e da SaúdeUniversidade do MinhoBragaPortugal
  2. 2.CIBIO/InBio—Centro de Investigação em Biodiversidade e Recursos GenéticosUniversidade do PortoVairãoPortugal
  3. 3.Grupo Lobo—Faculdade de Ciências da Universidade de LisboaLisbonPortugal
  4. 4.CBA—Centro de Biologia AmbientalFaculdade de Ciências da Universidade de LisboaLisbonPortugal
  5. 5.Associação ALDEIA—Centro de Ecologia, Recuperação e Vigilância de Animais Selvagens (CERVAS)GouveiaPortugal
  6. 6.A.RE.NA, Asesores en Recursos Naturales S.L.LugoSpain
  7. 7.Research Unit of Biodiversity (UO/CSIC/PA)Oviedo UniversityMieresSpain
  8. 8.Grimsö Wildlife Research StationSwedish University of Agricultural Sciences (SLU)RiddarhyttanSweden