Abstract
In a controlled rhizotrone experiment, stable isotope tracers of Mg, Ca and K were applied directly to the rhizosphere of an oak seedling using a 2D-array of micro ceramic cups. Before starting isotope application the oak root induced a significant reduction of K+, Ca2+, Mg2+ and NO −3 in the soil solution of the rhizosphere, as well as an increase of Al3+. The effect of adding stable isotopes in the soil (soil solution and exchangeable cations) was mainly restricted to a distance of about 1 cm from the point of application. All stable isotopes were taken up by the oak seedling, especially Ca which according to leaf analysis was in the range of insufficiency. As expected, Ca showed low mobility in the phloem, resulting in a low percentage of label in the root tip as compared to other root segments. Our experiment proved, that in-situ application is an easy to handle tool for carrying out tracer studies in real soil.
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References
van den Burg J (1985) Foliar analysis for determination of tree nutrient status—a compilation of literature data. Institute for Forestry and Urban Ecology, Wageningen
van den Burg J (1990) Foliar analysis for determination of tree nutrient status—a compilation of literature data 1985–1989. Institute for Forestry and Urban Ecology, Wageningen
Chung HH, Kramer PJ (1975) Absorption of water and 32P through suberized and unsuberized roots of Loblolly Pine. Can J For Res 5:229–235
Claassen N, Hendriks L, Jungk A (1981) Erfassung der Mineralstoffverteilung im wurzelnahen Boden durch Autoradiographie. Z Pflanzenernähr Bodenk 144:306–316
Comerford NB, Smethurst PJ, Escamilla JA (1994) Nutrient uptake by woody root systems. New Zealand J Forest Sci 24:195–212
Dieffenbach A, Göttlein A, Matzner E (1997) In-situ soil solution chemistry in an acid forest soil as influenced by growing roots of Norway spruce (Picea abies [L.] Karst.). Plant Soil 192:57–61
Göttlein A, Blasek R (1996) Analysis of small volumes of soil solution by capillary electrophoresis. Soil Sci 161:705–715
Göttlein A, Hell U, Blasek R (1996) A system for microscale tensiometry and lysimetry. Geoderma 69:147–156
Göttlein A, Heim A, Matzner E (1999) Mobilization of aluminium in the rhizosphere soil solution of growing tree roots in an acidic soil. Plant Soil 211:41–49
Häussling M, Jorns CA, Lehmbecker G, Hecht-Buchholz C, Marschner H (1988) Ion and water uptake in relation to root development in Norway spruce (Picea abies (L.) Karst.). J Plant Physiol 133:486–491
Kirlew PW, Bouldin DR (1987) Chemical properties of the rhizosphere of an acid subsoil. Soil Sci Soc Am J 51:128–132
Kuchenbuch R, Claassen N, Jungk A (1986) Potassium availability in relation to soil moisture II. Calculations by means of a mathematical simulation model. Plant Soil 95:233–243
Kuhn AJ, Bauch J, Schröder WH (1995) Monitoring uptake and contents of Mg, Ca, K in Norway spruce as influenced by pH and Al, using microprobe analysis and stable isotope labelling. Plant Soil 168–169:135–150
Kuhn AJ, Schröder WH, Bauch J (2000) The kinetics of calcium and magnesium entry into mycorrhized spruce roots. Planta 210:488–496
Larcher W (1994) Ökophysiologie der Pflanzen, 5th edn. Ulmer Verlag, Stuttgart
Manderscheid B, Göttlein A (eds) (1995) Wassereinzugsgebiet ’Lehstenbach’ - das BITÖK-Untersuchungsgebiet am Waldstein (Fichtelgebirge, NO-Bayern). Bayreuther Forum Ökologie, vol. 18, University of Bayreuth
Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic, London
Nye PH, Marriott FHC (1969) A theoretical study of the distribution of substances around roots resulting from simultaneous diffusion and mass flow. Plant Soil 30:459–472
Schachtschabel P, Blume HP, Hartge KH, Renger M (1982) Lehrbuch der Bodenkunde, 11th edn. Enke Verlag, Stuttgart
Schaller G, Fischer WR (1985) pH-Änderungen in der Rhizosphäre von Mais- und Erdnußwurzeln. Z Pflanzenernähr Bodenk 148:306–320
STMELF (1987) Grundsätze für die Düngung im Wald. Bayerisches Staatsministerium für Ernährung Landwirtschaft und Forsten. München
Wang Z, Göttlein A, Bartonek G (2001) Effects of growing roots of Norway spruce (Picea abies [L.] Karst.) and European beech (Fagus sylvatica L.) on rhizosphere soil solution. J Plant Nutr Soil Sci 164:35–41
Acknowledgements
The experiments were run at the chair of Soil Ecology, University of Bayreuth. We would like to thank Prof. E. Matzner for supporting our work and Mr. R. Blasek for technical assistance.
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Göttlein, A., Heim, A., Kuhn, A.J. et al. In-situ application of stable isotope tracers in the rhizosphere of an oak seedling. Eur J Forest Res 124, 83–86 (2005). https://doi.org/10.1007/s10342-005-0060-z
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DOI: https://doi.org/10.1007/s10342-005-0060-z