Skip to main content
SpringerLink
Log in

Reversed Phase Chromatographic Analysis of 13 Amino Acids in Honey Samples

  • Short Communication
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A simple method using reversed phase high-performance liquid chromatography (RP-HPLC) was developed for the simultaneous analysis of 13 amino acids. Amino acids were pre-column derivatized with 9-fluorenylmethyl chloroformate (FMOC-Cl) before analysis by RP-HPLC. Experimental parameters affecting the derivatization and chromatographic separation were investigated. Amino acids were derivatized with FMOC-Cl under alkaline condition in 0.1 mol/L borate buffer pH 10.0 at room temperature. The FMOC-amino acid derivatives were separated on an Atlantis C18 column under the gradient elution of 0.05 % trifluoroacetic acid and acetonitrile and UV detection at 265 nm. Linear ranges were 0.2–100.0 μg/mL with the correlation coefficients greater than 0.992. Limits of detection and limits of quantitation were in the range of 0.05–2.0 and 0.2–5.0 µg/L, respectively. The intra-day precision (n = 3) of retention time was less than 1 %, while for the peak area was less than 4 %. The inter-day precision (n = 3 × 3) of retention time was less than 2 % and the peak area was less than 8 %. This method was applied in honey samples and the results showed that proline is the major amino acids in honey samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

References

  1. Bradbear N (2004) Beekeeping and sustainable livelihoods. Food and Agriculture Organization of the United Nations, Rome

    Google Scholar 

  2. Boonchiangma S, Chanthai S, Srijaranai S, Srijaranai S (2011) J Food Process Eng 34:1584–1596

    Article  CAS  Google Scholar 

  3. Hermosín I, Chicón RM, Cabezudo MD (2003) Food Chem 83:263–268

    Article  Google Scholar 

  4. Fischer K, Chodura A, Kotalik J, Bieniek D, Kettrup A (1997) J Chromatogr A 770:228–241

    Article  Google Scholar 

  5. Hanko VP, Rohrer JS (2004) Anal Biochem 324:29–38

    Article  CAS  Google Scholar 

  6. Naval MV, Gómez-Serranillos MP, Carretero ME, Arce C (2006) J Chromatogr A 1121:242–247

    Article  CAS  Google Scholar 

  7. Bank RA, Jansen EJ, Beekman B, Koppele JMT (1996) Anal Biochem 240:167–176

    Article  CAS  Google Scholar 

  8. Spanik I, Horvathova G, Janocova A, Krupcik J (2007) J Chromatogr A 1150(145):154

    Google Scholar 

  9. Pappa-Louisi A, Nikitas P, Agrafiotou P, Papageorgiou A (2007) Anal Chim Acta 593:92–97

    Article  CAS  Google Scholar 

  10. Frank MP, Powers RW (2007) J Chromatogr B 852:646–649

    Article  CAS  Google Scholar 

  11. Bauza T, Kelly MT, Blaise A (2007) Food Chem 105:405–413

    Article  CAS  Google Scholar 

  12. Shangguan D, Zhao Y, Han H, Zhao R, Liu G (2001) Anal Chem 73:2054–2057

    Article  CAS  Google Scholar 

  13. Bernal JL, Nozal MJ, Toribio L, Diego JC, Ruiz A (2005) J Sep Sci 28:1039–1047

    Article  CAS  Google Scholar 

  14. Brückner H, Lüpke M (1995) J Chromatogr A 697:295–307

    Article  Google Scholar 

  15. Jámbor A, Molnár-Perl I (2009) J Chromatogr A 1216:3064–3077

    Article  Google Scholar 

  16. Chang X, Yu Z (2011) J Braz Chem Soc 22:1246–1252

    CAS  Google Scholar 

  17. Neuman RC (2013) Organic chemistry: chapter 22 peptides, proteins, and α-amino acids. The University of California, Riverside (UCR), California

    Google Scholar 

  18. Habib HM, Habib HM, Al Meqbali FT, Kamal H, Souka UD, Ibrahim WH (2014) Food Chem 153:35–43

    Article  CAS  Google Scholar 

  19. Pereira V, Pontes M, Câmara JS, Marques JC (2008) J Chromatogr A 1189:435–443

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Financial support from the Center of Excellence for Innovation in Chemistry (PERCH-CIC), Commission on Higher Education, Ministry of Education, Thailand and the Thailand Research Fund (TRF) through the Royal Golden Jubilee (RGJ) Ph.D. Program (Grant No. PHD/0354/2551) to Suthasinee Boonchiangma and Supalax Srijaranai are gratefully acknowledged.

Author information

Authors and Affiliations

  1. Department of Chemistry and Center of Excellence for Innovaiton in Chemistry, Materials Chemistry Research Center, Faculty of Science, Khon Kaen University, Mittraphap Rd, Khon Kaen, 40002, Thailand

    Suthasinee Boonchiangma, Patita Ratchakrut, Saksit Chanthai & Supalax Srijaranai

Authors
  1. Suthasinee Boonchiangma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Patita Ratchakrut
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Saksit Chanthai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Supalax Srijaranai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Supalax Srijaranai.

Additional information

Published in the topical collection 20th International Symposium on Separation Sciences in Prague with guest editors Aleš Horna and Pavel Jandera.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Boonchiangma, S., Ratchakrut, P., Chanthai, S. et al. Reversed Phase Chromatographic Analysis of 13 Amino Acids in Honey Samples. Chromatographia 78, 923–927 (2015). https://doi.org/10.1007/s10337-015-2894-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10337-015-2894-y

Keywords

Search

Navigation