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Developments in Interfacing Designs for CE–MS: Towards Enabling Tools for Proteomics and Metabolomics

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Abstract

Capillary electrophoresis–mass spectrometry (CE–MS) can be considered a useful analytical technique for the analysis of charged compounds in the fields of proteomics and metabolomics. Currently, the commercially available co-axial sheath–liquid interface is generally employed for coupling CE to MS in most application areas. Although it has proven to be rather robust for various proteomics, glycomics and metabolomics studies, the intrinsically low-flow separation property of CE is not effectively utilized in this set-up. In this type of interfacing the sheath–liquid (typical flow-rate between 1 and 10 µL/min) dilutes the CE effluent (flow-rate between 20 and 100 nL/min), thereby reducing the detection sensitivity. Over the past few years some significant developments that aim to overcome this limitation have been made in interfacing techniques for CE–MS, which resulted in an increased interest of CE–MS for proteomics and metabolomics. This paper provides an overview of these developments and the utility of CE–MS employing the new interfacing techniques is demonstrated by representative examples in the fields of proteomics, glycomics and metabolomics. Finally, general conclusions and perspectives are provided.

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Acknowledgments

Dr. Rawi Ramautar would like to acknowledge the financial support of the Veni grant scheme of the Netherlands Organization of Scientific Research (NWO Veni 722.013.008).

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Authors and Affiliations

  1. Division of Analytical Biosciences, Leiden Academic Center for Drug Research (LACDR), Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands

    Petrus W. Lindenburg & Rawi Ramautar

  2. Division of BioAnalytical Chemistry, AIMMS Research Group BioMolecular Analysis, VU University Amsterdam, De Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands

    Rob Haselberg

  3. ROZING.COM Consulting, Gerberastrasse 2, 76228, Karlsruhe, Germany

    Gerard Rozing

Authors
  1. Petrus W. Lindenburg
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  2. Rob Haselberg
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  3. Gerard Rozing
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  4. Rawi Ramautar
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Corresponding author

Correspondence to Rawi Ramautar.

Additional information

Published in the topical collection Recent Developments in Clinical Omics with guest editors Martin Giera and Manfred Wuhrer.

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Lindenburg, P.W., Haselberg, R., Rozing, G. et al. Developments in Interfacing Designs for CE–MS: Towards Enabling Tools for Proteomics and Metabolomics. Chromatographia 78, 367–377 (2015). https://doi.org/10.1007/s10337-014-2795-5

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  • DOI: https://doi.org/10.1007/s10337-014-2795-5

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