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Interaction of E6 Gene from Human Papilloma Virus 16 (HPV-16) with CdS Quantum Dots

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Abstract

The aim of this study was to analyze the interactions of blue and yellow fluorescent CdS quantum dots (CdS-QDs) with human papillomavirus 16 (HPV-16) oncogene E6. The interactions were investigated using chip capillary electrophoresis, spectrophotometry and square wave voltammetry (SWV). Using chip capillary electrophoresis we proved that blue fluorescent CdS-QDs (0.5 mM) caused an increase of the migration time of the E6 HPV-16 DNA–CdS-QDs complex by 42 s compared to control DNA (E6 HPV-16). The same concentration of yellow fluorescent CdS-QDs caused an increase in the migration time of the DNA–CdS-QDs complex by 108 s compared to the control DNA (E6 HPV-16). The difference in the migration times between both complexes was 66 s. Using square wave voltammetry (SWV), the reduction signal of cytosine and adenine (peak CA) was observed, after the complex with 2.5 µg mL−1 DNA was formed. A decrease of the peak CA reduction signal of the complex DNA–CdS-QDs by 90 % was caused when yellow fluorescent CdS-QDs (0.03 mM) were used. The same concentration of blue fluorescent CdS-QDs caused only a 50 % decrease of the C and A reduction signal of the DNA–CdS-QDs complex. The difference between both CdS-QDs was 40 %. Electrochemical measurements and chip electrophoresis analyses confirmed that the yellow fluorescent CdS-QDs show higher affinity to the DNA (E6 HPV-16) compared to blue ones.

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Acknowledgments

The authors are grateful to CEITEC CZ.1.05/1.1.00/02.0068 for financial support. The authors wish to thank also Ana Jimenez Jimenez for technical assistance.

Conflict of interest

The authors have declared no conflict of interest.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic

    Lukas Nejdl, Sylvie Skalickova, Jiri Kudr, Branislav Ruttkay-Nedecky, Hoai Viet Nguyen, Miguel Angel Merlos Rodrigo, Pavel Kopel, Marie Konecna, Vojtech Adam & Rene Kizek

  2. Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, 616 00, Brno, Czech Republic

    Lukas Nejdl, Sylvie Skalickova, Jiri Kudr, Branislav Ruttkay-Nedecky, Hoai Viet Nguyen, Miguel Angel Merlos Rodrigo, Pavel Kopel, Marie Konecna, Vojtech Adam & Rene Kizek

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  1. Lukas Nejdl
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  2. Sylvie Skalickova
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  3. Jiri Kudr
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  4. Branislav Ruttkay-Nedecky
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  5. Hoai Viet Nguyen
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  6. Miguel Angel Merlos Rodrigo
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  7. Pavel Kopel
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  8. Marie Konecna
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  9. Vojtech Adam
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  10. Rene Kizek
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Corresponding author

Correspondence to Rene Kizek.

Additional information

Published in the topical collection Advances in Chromatography and Electrophoresis & Chiranal 2014 with guest editor Jan Petr.

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Nejdl, L., Skalickova, S., Kudr, J. et al. Interaction of E6 Gene from Human Papilloma Virus 16 (HPV-16) with CdS Quantum Dots. Chromatographia 77, 1433–1439 (2014). https://doi.org/10.1007/s10337-014-2734-5

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  • DOI: https://doi.org/10.1007/s10337-014-2734-5

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