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Tetrazole-Functionalized Silica for Hydrophilic Interaction Chromatography of Polar Solutes

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Abstract

In this work, tetrazole-functionalized stationary phase was prepared with nitrile-modified silica by an ammonium-catalyzed (3 + 2) azide-nitrile cycloaddition reaction. The prepared stationary phase was used for separation of nucleobases and nucleosides by hydrophilic interaction chromatography (HILIC) mode. A typical HILIC mechanism was observed at higher content of acetonitrile (>85%, v/v) in the mobile phase. The retention mechanism of the column was investigated by the models used for describing partitioning and surface adsorption through adjustment ratio of water in the mobile phase, and by the influence of salt concentration, buffer pH, and temperature on the retention of solutes. The results illustrated that the surface adsorption through hydrogen bonding dominated the retention behavior of nucleobases/nucleosides under HILIC mode. From the separation ability, the tetrazole-functionalized stationary phase could become a valuable alternative for the separation of the compounds concerned.

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References

  1. Alpert AJ (1990) J Chromatogr 499:177–196

    Article  CAS  Google Scholar 

  2. Van Dorpe S, Vergote V, Pezeshki A, Burvenich C, Peremans K, De Spiegeleer B (2010) J Sep Sci 33:728–739

    Article  Google Scholar 

  3. Ahn J, Bones J, Yu YQ, Rudd PM, Gilar M (2010) J Chromatogr B 878:403–408

    Article  CAS  Google Scholar 

  4. Ihunegbo FN, Tesfalidet S, Jiang W (2010) J Sep Sci 33:988–995

    Article  Google Scholar 

  5. Shi ZG, Wu YB, Feng YQ, Luo YB (2010) Chromatographia 71:761–768

    Article  CAS  Google Scholar 

  6. Dejaegher B, Heyden YV (2010) J Sep Sci 33:698–715

    Article  CAS  Google Scholar 

  7. Kalili KM, de Villiers A (2009) J Chromatogr A 1216:6274–6284

    Article  CAS  Google Scholar 

  8. Hemström P, Irgum K (2006) J Sep Sci 29:1784–1821

    Article  Google Scholar 

  9. Jandera P, Hájek T (2009) J Sep Sci 32:3603–3619

    Article  CAS  Google Scholar 

  10. Jandera P, Hájek T, Škeříková V, Soukup J (2010) J Sep Sci 33:841–852

    Article  CAS  Google Scholar 

  11. Yanagida A, Murao H, Ohnishi-Kameyama M, Yamakawa Y, Shoji A, Tagashira M, Kanda T, Shibusawa Y (2007) J Chromatogr A 1143:153–161

    Article  CAS  Google Scholar 

  12. Guo ZM, Lei AW, Zhang YP, Xu Q, Xue XY, Zhang FF, Liang XM (2007) Chem Comm 24:2491–2493

    Article  Google Scholar 

  13. Fu Q, Guo ZM, Liang T, Zhang XL, Xu Q, Liang XM (2010) Anal Methods 2:217–224

    Article  CAS  Google Scholar 

  14. Guo ZM, Jin Y, Liang T, Liu YF, Xu Q, Liang XM, Lei AW (2009) J Chromatogr A 1216:257–263

    Article  CAS  Google Scholar 

  15. Moni L, Ciogli A, D’Acquarica I, Dondoni A, Gasparrini F, Marra A (2010) Chem Eur J 16:5712–5722

    CAS  Google Scholar 

  16. Huang HX, Jin Y, Xue MY, Yu L, Fu Q, Ke YX, Chu CH, Liang XM (2009) Chem Comm 45:6973–6975

    Article  Google Scholar 

  17. Jason HR (2002) Bioorg Med Chem 10:3379–3393

    Article  Google Scholar 

  18. Lei GH, Xiong XH, Huo YM, Wei YM, Zheng XH (2008) Chem J Chinese Univ 29:268–272

    CAS  Google Scholar 

  19. Lei GH, Xiong XH, Huo YM, Wei YM, Zheng XH, Zheng JB (2008) J Chromatogr A 1187:197–204

    Article  CAS  Google Scholar 

  20. Trifonov RE, Alkorta I, Ostrovskiia VA, Elguero J (2004) J Mol Struct (Theochem) 668:123–132

    Article  CAS  Google Scholar 

  21. Marrubini G, Castillo Mendoza BE, Massolini G (2010) J Sep Sci 33:803–816

    Article  CAS  Google Scholar 

  22. Li RP, Huang JX (2004) J Chromatogr A 1041:163–169

    Article  CAS  Google Scholar 

  23. Wu JY, Bicker W, Lindner W (2008) J Sep Sci 31:1492–1503

    Article  CAS  Google Scholar 

  24. Nguyen HP, Yang SH, Wigginton JG, Simpkins JW, Schug KA (2010) J Sep Sci 33:793–802

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by National Natural Science Foundation of China (Nos. 20575052 and 20975080), Program for New Century Excellent Talents in University (No. NCET-08-0892) and Natural Science Foundation of Ningxia Province (No.NZ0914).

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Correspondence to Yinmao Wei.

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Dai, X., Qian, X., Gong, B. et al. Tetrazole-Functionalized Silica for Hydrophilic Interaction Chromatography of Polar Solutes. Chromatographia 73, 865–870 (2011). https://doi.org/10.1007/s10337-011-1985-7

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  • DOI: https://doi.org/10.1007/s10337-011-1985-7

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