Abstract
In this work, tetrazole-functionalized stationary phase was prepared with nitrile-modified silica by an ammonium-catalyzed (3 + 2) azide-nitrile cycloaddition reaction. The prepared stationary phase was used for separation of nucleobases and nucleosides by hydrophilic interaction chromatography (HILIC) mode. A typical HILIC mechanism was observed at higher content of acetonitrile (>85%, v/v) in the mobile phase. The retention mechanism of the column was investigated by the models used for describing partitioning and surface adsorption through adjustment ratio of water in the mobile phase, and by the influence of salt concentration, buffer pH, and temperature on the retention of solutes. The results illustrated that the surface adsorption through hydrogen bonding dominated the retention behavior of nucleobases/nucleosides under HILIC mode. From the separation ability, the tetrazole-functionalized stationary phase could become a valuable alternative for the separation of the compounds concerned.
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Acknowledgments
This work was supported by National Natural Science Foundation of China (Nos. 20575052 and 20975080), Program for New Century Excellent Talents in University (No. NCET-08-0892) and Natural Science Foundation of Ningxia Province (No.NZ0914).
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Dai, X., Qian, X., Gong, B. et al. Tetrazole-Functionalized Silica for Hydrophilic Interaction Chromatography of Polar Solutes. Chromatographia 73, 865–870 (2011). https://doi.org/10.1007/s10337-011-1985-7
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DOI: https://doi.org/10.1007/s10337-011-1985-7