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Characterization of Alternaria species associated with muskmelon foliar diseases in Beijing municipality of China

  • Fungal Diseases
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Abstract

A total of 41 Alternaria isolates was recovered from diseased muskmelon leaves with foliar disease symptoms, which were collected from Beijing municipality of China. Thirty-one isolates were identified as Alternaria tenuissima and 10 isolates as A. alternata based on morphological traits, ITS and histone 3 nucleotide sequences. The Alternaria isolates induced the original symptoms including chlorosis and brown lesions on detached muskmelon leaves, with disease incidence ranging from 33.3 to 87.5 %. No significant differences in disease index were detected between the two species. This is the first report of A. tenuissima causing foliar diseases on muskmelon plants in Beijing.

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Acknowledgements

This work was supported by Beijing Innovation Consortium of Melon Research System. Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement.

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Correspondence to Xuehong Wu.

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Supplementary material 1 (DOC 61 kb)

10327_2015_631_MOESM2_ESM.tif

Supplementary Fig. 1 Phylogenetic tree constructed based on the sequences of the ITS region of rDNA of 31 A. tenuissima isolates, 10 A. alternata isolates, and 8 reference sequences retrieved from GenBank. The tree was constructed by neighbor joining using the Kimura two-parameter distance method. Bootstrap values (in percentage) above 70 from 1000 pseudo-replicates are shown for major lineages within the tree. The marker denotes a measurement of relative phylogenetic distance

10327_2015_631_MOESM3_ESM.tif

Supplementary Fig. 2 Phylogenetic tree constructed based on the partial coding sequences of histone 3 gene consisting of 31 A. tenuissima isolates, 10 A. alternata isolates, and 9 reference sequences retrieved from GenBank. The tree was constructed by neighbor joining using the Kimura two-parameter distance method. Bootstrap values (in percentage) above 70 from 1000 pseudo-replicates are shown for major lineages within the tree. The marker denotes a measurement of relative phylogenetic distance

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Zhao, J., Bao, S., Ma, G. et al. Characterization of Alternaria species associated with muskmelon foliar diseases in Beijing municipality of China. J Gen Plant Pathol 82, 29–32 (2016). https://doi.org/10.1007/s10327-015-0631-x

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  • DOI: https://doi.org/10.1007/s10327-015-0631-x

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