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Detection and quantification of onion isolates of Fusarium oxysporum f. sp. cepae in onion plant

  • Fungal Diseases
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

Fusarium oxysporum f. sp. cepae (FOC) isolated from onion basal rot possessed a SIX3 homolog (FocSIX3) with 91.4 % identity to the SIX3 gene (FolSIX3) of F. oxysporum f. sp. lycopersici (FOL). A primer pair (P1) was designed to detect onion FOC based on differences in nucleotide sequences between FocSIX3 and FolSIX3. P1 amplified a 106-bp-long DNA segment only from FOC isolates pathogenic to onions, but not from FOC isolated from either Welsh onion or F. oxysporum strains belonging to formae speciales other than cepae. Real-time quantitative PCR with P1 was developed to quantify onion root- and basal-plate-colonizing FOC.

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Acknowledgments

This work was supported by a Grant-in-Aid for Scientific Research (C) (23580063) from the Japanese Society for Promotion of Sciences (JSPS). We thank Mr. Hidetsugu Nishihata (Toyama Prefectural Agricultural, Forestry and Fisheries Research Center) and Mr. Atsuoki Sue (Takii & Company Ltd.) for kindly providing onion samples. We thank Dr. F. Namiki for providing F. oxysporum f. sp. lycopersici strain CK3-1.

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Authors and Affiliations

  1. The United Graduate School of Agricultural Sciences, Tottori University, Tottori, 680-8553, Japan

    Kazunori Sasaki

  2. Department of Biological and Environmental Sciences, Faculty of Agriculture, Yamaguchi University, Yamaguchi, 753-8515, Japan

    Katsuya Nakahara, Masayoshi Shigyo, Shuhei Tanaka & Shin-ichi Ito

Authors
  1. Kazunori Sasaki
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  2. Katsuya Nakahara
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  3. Masayoshi Shigyo
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  4. Shuhei Tanaka
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  5. Shin-ichi Ito
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Corresponding author

Correspondence to Shin-ichi Ito.

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Sasaki, K., Nakahara, K., Shigyo, M. et al. Detection and quantification of onion isolates of Fusarium oxysporum f. sp. cepae in onion plant. J Gen Plant Pathol 81, 232–236 (2015). https://doi.org/10.1007/s10327-015-0589-8

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  • DOI: https://doi.org/10.1007/s10327-015-0589-8

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