Abstract
To improve the diagnosis of Stewart’s wilt, we developed a loop-mediated isothermal amplification (LAMP) assay, based on two conserved sequences of the cpsD and pstS–glmS gene regions. The detection limit of the LAMP assay was 104 colony-forming units/mL. No cross reaction was observed with other Pantoea spp., other genera associated with sweet corn diseases, or strains isolated from the surface of maize leaves in Japan. The LAMP reaction was not inhibited by leaf contents. The simplicity of sample preparation and short processing time make this LAMP assay useful for field surveillance of Stewart’s wilt.
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We thank the National Institute of Agrobiological Sciences for providing the bacterial strains.
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Uematsu, H., Inoue, Y. & Ohto, Y. Detection of Pantoea stewartii from sweet corn leaves by loop-mediated isothermal amplification (LAMP). J Gen Plant Pathol 81, 173–179 (2015). https://doi.org/10.1007/s10327-015-0580-4
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DOI: https://doi.org/10.1007/s10327-015-0580-4