Abstract
We tested citrus samples from Tunisia using reverse transcription-polymerase chain reaction (RT-PCR), and for the first time, Citrus viroid V (CVd-V) was reported in North Africa. Fourteen of 38 tested citrus trees were infected by CVd-V including the majority of varieties grown in Tunisia. Some RT-PCR results were also supported by biological indexing. After sequencing the RT-PCR products, three new CVd-V variants were identified, showing 80–91 % nucleotide sequence identity with those reported previously. Based on phylogenetic analysis using all CVd-V sequences in GenBank, two main CVd-V groups were identified. Furthermore, construction of a genetic network of the detected haplotypes using the same sequences shows a clear geographical structuring of Tunisian CVd-V variants.
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Acknowledgments
This work was supported by grants from the Tunisian “Ministère de l’Enseignement supérieur et de la recherche Scientifique”. The authors would like to acknowledge P. Serra and N. Duran-Vila for providing the positive control and M. Eiras for providing the CVd-V probe.
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Hamdi, I., Elleuch, A., Bessaies, N. et al. First report of Citrus viroid V in North Africa. J Gen Plant Pathol 81, 87–91 (2015). https://doi.org/10.1007/s10327-014-0556-9
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DOI: https://doi.org/10.1007/s10327-014-0556-9