Skip to main content
SpringerLink
Log in

First report of broccoli foot rot caused by Rhizoctonia solani AG-2-2 IV and pathogenicity comparison of the pathogen with related pathogens

  • Fungal Diseases
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

Foot rot and wilt symptoms were found on mature broccoli plants in Hokkaido, Japan, in 2007. The causal fungus was identified as Rhizoctonia solani anastomosis group (AG)-2-2 IV based on a hyphal fusion test, cultural characteristics and DNA analysis. We compared the isolate with two reference isolates, the pathogen of broccoli damping-off, belonging to AG-1 IC and AG-2-2 IIIB, in terms of differences in virulence to broccoli plants at different growth stages under various conditions. All the isolates caused damping-off on all three broccoli cultivars tested. However, only the present isolate incited foot rot with wilt symptoms on mature plants, when temperature was optimum for plant growth (20–25 °C), indicating that the foot rot pathogen may be discriminated from the damping-off pathogen in terms of virulence to mature broccoli. We also compared the isolate with 10 reference isolates from various crops, belonging to AG-2-2 IV, AG-2-2 IIIB and AG-1 IC, in terms of virulence to broccoli. All the isolates caused damping-off on broccoli seedlings with one exception; however, only the present isolate and one of four AG-2-2 IV isolates tested caused foot rot with wilt symptoms on mature broccoli in all the replicates. These results suggest that a group of isolates among AG-2-2 IV population is capable of causing foot rot on mature broccoli plants. This is the first report of broccoli foot rot caused by R. solani AG-2-2 IV.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Arai T, Tujii O, Ohira J, Tohyama Y, Ohnishi H, Yamamoto M, Kobayashi N, Nemoto K, Yashiro K (2010) Production cases of broccoli (in Japanese). In: Editing team of Noukou-to-Engei (eds) Physiological ecology and production cases of broccoli. Seibundo-Shinkosya, Tokyo, pp 114–181

  • Björkman T, Pearson KJ (1998) High temperature arrest of inflorescence development in broccoli (Brassica oleracea var. italica L.). J Exp Bot 49:101–106

    Article  Google Scholar 

  • Budge GE, Shaw MW, Lambourne C, Jennings P, Clayburn R, Boonham N, McPherson M (2009) Characterization and origin of infection of Rhizoctonia solani associated with Brassica oleracea crops in the UK. Plant Pathol 58:1059–1070

    Article  CAS  Google Scholar 

  • Carling DE (1996) Grouping in Rhizoctonia solani by hyphal anastomosis reaction. In: Sneh B, Jabaji-Hare S, Neate S, Dijst G (eds) Rhizoctonia species: taxonomy, molecular biology, ecology, pathology and disease control. Kluwer, Dordrecht, pp 37–47

    Google Scholar 

  • Carling DE, Kuninaga S, Brainard KA (2002) Hyphal anastomosis reactions, rDNA-internal transcribed spacer sequences, and virulence levels among subsets of Rhizoctonia solani anastomosis group-2 (AG-2) and AG-BI. Phytopathology 92:43–50

    Article  CAS  PubMed  Google Scholar 

  • Eimori K, Shimada R, Takeuchi J (2005) First occurrence of bottom rot of Brassica campestris L. (rapifera group) and damping-off of Raphanus sativus L. (daikon group) caused by Rhizoctonia solani in Japan (in Japanese). Annu Rep Kanto-Tosan Plant Prot Soc 52:35–38

    Google Scholar 

  • Fujime H (2010) Physiological ecology of broccoli (in Japanese). In: Editing team of Noukou-to-Engei (eds) Physiological ecology and production cases of broccoli. Seibundo-Shinkosya, Tokyo, pp 8–112

  • Homma Y, Ishii M (1984) Anastomosis groups of Rhizoctonia solani Kühn responsible for various symptoms of the root rot of Japanese radish (in Japanese with English summary). Bull Shikoku Agric Exp Stn 42:1–11

    Google Scholar 

  • Kubota M, Abiko K (1997) Rhizoctonia solani Kühn isolated from damping-off broccoli (in Japanese). Proc Kansai Pl Prot 39:33–34

    Google Scholar 

  • Kubota M, Tomioka K, Sato T (2009) Damping-off of broccoli caused by Rhizoctonia solani AG-1 IC (in Japanese). Annu Rep Kansai Pl Prot 51:27–28

    Article  Google Scholar 

  • Kuninaga S (2003) Current situation of the taxonomy of Rhizoctonia solani (in Japanese). Plant Prot 57:219–222

    Google Scholar 

  • Kuramae EE, Buzeto AL, Ciampi MB, Souza NL (2003) Identification of Rhizoctonia solani AG 1-IB in lettuce, AG 4 HG-I in tomato and melon, and AG 4 HG-III in broccoli and spinach, in Brazil. Eur J Pl Pathol 109:391–395

    Article  CAS  Google Scholar 

  • Lipman DJ, Pearson WR (1985) Rapid and sensitive protein similarity searches. Science 227:1435–1441

    Article  CAS  PubMed  Google Scholar 

  • Martin B (1987) Rapid tentative identification of Rhizoctonia spp. associated with diseased turfgrasses. Plant Dis 71:47–49

    Article  Google Scholar 

  • McCarter SM (1991) Rhiozoctonia diseases. In: Jones JB, Jones JP, Stall RE, Zitter TA (eds) Compendium of tomato diseases. APS Press, St. Paul, pp 21–22

    Google Scholar 

  • Misawa T, Kuninaga S (2010) The first report of tomato foot rot caused by Rhizoctonia solani AG-3 PT and AG-2-Nt and its host range and molecular characterization. J Gen Plant Pathol 76:310–319

    Article  Google Scholar 

  • Misawa T, Kuninaga S (2013) First report of white leaf rot on Chinese chives caused by Rhizoctonia solani AG-2-1. J Gen Plant Pathol 79:280–283

    Article  CAS  Google Scholar 

  • Misawa T, Yamazaki K, Takada K (2013) Damping-off of broccoli caused by Rhizoctonia solani AG-2-1 (in Japanese with English summary). Annu Rep Plant Prot North Jpn 64:60–64

    Google Scholar 

  • Nagai Y, Fukatsu R (1971) Root rot symptom of carrot caused by Rhizoctonia solani (abstract in Japanese). Ann Phytopath Soc Japan 37:369

    Google Scholar 

  • Nakayama K, Ishikawa S, Masuhara G (1992) Occurrence of Chinese chive leaf rot by Rhizoctonia solani (in Japanese). Plant Prot 46:64–65

    Google Scholar 

  • Ogoshi A (1976) Studies on the grouping of Rhizoctonia solani Kühn with hyphal anastomosis and on the perfect stages of groups (in Japanese with English summary). Bull Nat Inst Agric Sci Ser C 30:1–63

    Google Scholar 

  • Ogoshi A (1987) Ecology and pathogenicity of anastomosis and intraspecific groups of Rhizoctonia solani Kühn. Annu Rev Phytopathol 25:125–143

    Article  Google Scholar 

  • Sneh B, Burpee L, Ogoshi A (1991) Identification of Rhizoctonia species. APS Press, St. Paul, pp 17–18

    Google Scholar 

  • Takano K, Toyota K (1985) Occurrence of leaf blight of Japanese radish in greenhouse (in Japanese). Proc Assoc Plant Prot Hokuriku 33:96–99

    Google Scholar 

  • Watanabe B, Matsuda A (1966) Studies on grouping Rhizoctonia solani Kühn pathogenic to upland crops (in Japanese with English summary). Bull Appointed Exp 7:1–131

  • White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322

    Chapter  Google Scholar 

  • Yamauchi N, Miura Y, Shirakawa T (2009) Damping-off of broccoli caused by Rhizoctonia solani AG-4 HG-I (in Japanese with English summary). Annu Rep Plant Prot North Jpn 60:105–107

    Google Scholar 

  • Yonemoto K, Hirota K, Hyakumachi M (2008) Seedling damping-off of carrot caused by Rhizoctonia solani AG-4 HG-II (in Japanese with English summary). Jpn J Phytopathol 74:20–23

    Article  Google Scholar 

Download references

Acknowledgments

We thank Dr. N. Matsumoto (Hokkaido University, Japan) for valuable comments and English correction of the manuscript and Y. Kishida (Hokkaido Prefectural Hiyama Agricultural Extension Center, Japan) for sampling support.

Author information

Authors and Affiliations

  1. Donan Agricultural Experiment Station, Hokkaido Research Organization, Hokuto, Hokkaido, 041-1201, Japan

    Tomoo Misawa

  2. National Agriculture and Food Research Organization, Institute of Vegetable and Tea Science, Tsukuba, Ibaraki, 305-8666, Japan

    Masaharu Kubota

  3. Kitami Agricultural Experiment Station, Hokkaido Research Organization, Kun-Neppu, Hokkaido, 099-1496, Japan

    Jun Sasaki

  4. Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan

    Shiro Kuninaga

Authors
  1. Tomoo Misawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masaharu Kubota
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jun Sasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shiro Kuninaga
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tomoo Misawa.

Additional information

The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases as accessions AB911320–911323.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Misawa, T., Kubota, M., Sasaki, J. et al. First report of broccoli foot rot caused by Rhizoctonia solani AG-2-2 IV and pathogenicity comparison of the pathogen with related pathogens. J Gen Plant Pathol 81, 15–23 (2015). https://doi.org/10.1007/s10327-014-0551-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10327-014-0551-1

Keywords

Search

Navigation