Skip to main content
SpringerLink
Log in

Effect of acibenzolar-S-methyl, strobilurins and other fungicide treatments on phylloplane fungi of crops

  • Others
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

Strobilurins, broad-spectrum fungicides used to control various diseases, may also inhibit the growth of nontarget fungi in the natural environment. Acibenzolar-S-methyl (ASM) is a plant activator; however, the effect of ASM on the growth of microflora on the leaf surface is not clear yet. ASM, strobilurins and other fungicides were sprayed on Japanese pear and cucumber plants in the field. Variations in the morphological characteristics and rDNA-ITS nucleotide sequence of isolated phylloplane fungi were evaluated. The isolation frequency of Alternaria spp., Aureobasidium spp., Cladosporium spp. and Myrothecium spp. on Japanese pear leaves treated with kresoxim-methyl and polycarbamate was lower than on nontreated pear leaves. Moreover, the frequency of Alternaria spp., Cladosporium spp., Epicoccum spp. and Fusarium spp. on leaves of two cucumber cultivars treated with azoxystrobin, triflumizole and mancozeb was lower than the frequency of fungal isolation on nontreated cucumber leaves. The effect of ASM on fungal isolation frequency was not significantly different from that on nontreated pear or cucumber leaves. The fungal genera observed and isolation frequency on the leaves treated with polycarbamate, azoxystrobin, triflumizole and mancozeb tended to be reduced compared to the no-spray treatment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Andrew JH, Harris RF (2000) The ecology and biogeography of microorganisms on plant surfaces. Annu Rev Phytopathol 38:145–180

    Article  Google Scholar 

  • Andrews JH, Kenerley CM (1978) The effects of a pesticide program on non-target epiphytic microbial populations of apple leaves. Can J Microbiol 24:1058–1072

    Article  PubMed  CAS  Google Scholar 

  • Avila-Adame C, Olaya G, Köller W (2003) Characterization of Colletotrichum graminicola isolates resistant to strobilurin-related QoI fungicides. Plant Dis 87:1426–1432

    Article  CAS  Google Scholar 

  • Bartlett DW, Clough JM, Godwin JR, Hall AA, Hamer M, Parr-Dobrzanski B (2002) The strobilurin fungicides. Pest Manag Sci 58:649–662

    Article  PubMed  CAS  Google Scholar 

  • Benigni M, Bompeix G (2004) Control of Phytophthora cryptogea (Pethyb. and Laff.) of witloof chicory (Cichorium intybus L.) with azoxystrobin applied before the forcing period. Crop Prot 23:1011–1014

    Article  CAS  Google Scholar 

  • Cao J, Jiang W, He H (2005) Induced resistance in Yali pear (Pyrus bretschneideri Rehd.) fruit against infection by Penicillium expansum by postharvest infiltration of acibenzolar-S-methyl. J Phytopathol 153:640–646

    Article  CAS  Google Scholar 

  • Chung WH, Tsukiboshi T (2005) A new species of Curvularia from Japan. Mycotaxon 91:49–54

    Google Scholar 

  • Elmer WH (2006) Effects of acibenzolar-S-methyl on the suppression of Fusarium wilt of cyclamen. Crop Prot 25:671–676

    Article  CAS  Google Scholar 

  • Faessel L, Nassr N, Lebeau T, Walter B (2008) Effects of the plant defence inducer, acibenzolar-S-methyl, on hypocotyl rot of soybean caused by Rhizoctonia solani AG-4. J Phytopathol 156:236–242

    Article  CAS  Google Scholar 

  • Faize M, Faize L, Koike N, Ishizaka M, Ishii H (2004) Acibenzolar-S-methyl-induced resistance to Japanese pear scab is associated with potentiation of multiple defense responses. Phytopathology 94:604–612

    Article  PubMed  CAS  Google Scholar 

  • Färber RBK, Chin KM, Leadbitter N (2002) Sensitivity of Venturia inaequalis to trifloxystrobin. Pest Manag Sci 58:261–267

    Article  PubMed  Google Scholar 

  • Gisi U, Sierotzki H, Cook A, McCaffery A (2002) Mechanisms influencing the evolution of resistance to Qo inhibitor fungicides. Pest Manag Sci 58:859–867

    Article  PubMed  CAS  Google Scholar 

  • Goodwin DC, Lee SB (1993) Microwave miniprep of total genomic DNA from fungi, plants, protists and animals for PCR. Biotechniques 15:438–444

    PubMed  CAS  Google Scholar 

  • Grasso V, Palermo S, Sierotzki H, Garibaldi A, Gisi U (2006a) Cytochrome b gene structure and consequences for resistance to Qo inhibitor fungicides in plant pathogens. Pest Manag Sci 62:465–472

    Article  PubMed  CAS  Google Scholar 

  • Grasso V, Sierotzki H, Garibaldi A, Gisi U (2006b) Characterization of the cytochrome b gene fragment of Puccinia species responsible for the binding site of QoI fungicides. Pestic Biochem Physiol 84:72–82

    Article  CAS  Google Scholar 

  • Heaney SP, Hall AA, Davies SA, Olaya G (2000) Resistance to fungicides in the QoI-STAR cross-resistance group: current perspectives. In: Proc. BCPC conf.—pests and diseases, BCPC, Alton, Hants, UK, pp 755–762

  • Inada M, Ishii H, Chung WH, Yamada T, Yamaguchi J, Furuta A (2008) Occurrence of strobilurin-resistant strains of Colletotrichum gloeosporioides (Glomerella cingulata), the causal fungus of strawberry anthracnose (in Japanese). Jpn J Phytopathol 74:114–117

    Article  CAS  Google Scholar 

  • Ishii H, Tomita Y, Horio T, Narusaka Y, Nakazawa Y, Nishimura K, Iwamoto S (1999) Induced resistance of acibenzolar-S-methyl (CGA245704) to cucumber and Japanese pear diseases. Eur J Plant Pathol 105:77–85

    Article  CAS  Google Scholar 

  • Ishii H, Fraaije BA, Sugiyama T, Noguchi K, Nishimura K, Takeda T, Amano T, Hollomon DW (2001) Occurrence and molecular characterization of strobilurin resistance in cucumber powdery mildew and downy mildew. Phytopathology 91:1166–1171

    Article  PubMed  CAS  Google Scholar 

  • Ishii H, Fountaine J, Chung WH, Kansako M, Nishimura K, Takahashi K, Oshima M (2009) Characterisation of QoI-resistant field isolates of Botrytis cinerea from citrus and strawberry. Pest Manag Sci 65:916–922

    Article  PubMed  CAS  Google Scholar 

  • Kalinin VA, Bykov KV, Osman AG (2002) Effects of azoxystrobin on soil microorganisms under laboratory conditions. In: Proc. BCPC conf.—pests and diseases, BCPC, Alton, Hants, UK, pp 279–284

  • Langvad F (1980) A simple and rapid method for qualitative and quantitative study of the fungal flora of leaves. Can J Bot 26:666–670

    CAS  Google Scholar 

  • Lawton KA, Friedrich L, Hunt M, Weymann K, Delaney T, Kessmann H, Staub T, Ryals J (1996) Benzothiadiazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway. Plant J 10:71–82

    Article  PubMed  CAS  Google Scholar 

  • Leben C (1965) Epiphytic microorganisms in relation to plant diseases. Annu Rev Phytopathol 3:209–230

    Article  Google Scholar 

  • Ma Z, Felts D, Michailides TJ (2003) Resistance to azoxystrobin in Alternaria isolates from pistachio in California. Pestic Biochem Physiol 77:66–74

    Article  CAS  Google Scholar 

  • Markoglou AN, Malandrakis AA, Vitoratos AG, Ziogas BN (2006) Characterization of laboratory mutants of Botrytis cinerea resistant to QoI fungicides. Eur J Plant Pathol 115:149–162

    Article  CAS  Google Scholar 

  • Narusaka Y, Narusaka M, Horio T, Ishii H (1999a) Induction of disease resistance in cucumber by acibenzolar-S-methyl and expression of resistance-related genes. Ann Phytopathol Soc Jpn 65:116–122

    Article  CAS  Google Scholar 

  • Narusaka Y, Narusaka M, Horio T, Ishii H (1999b) Comparison of local and systemic induction of acquired disease resistance in cucumber plants treated with benzothiadiazoles or salicylic acid. Plant Cell Physiol 40:388–395

    Article  PubMed  CAS  Google Scholar 

  • Norse D (1972) Fungal populations of tobacco leaves and their effect on the growth of Alternaria longipes. Trans Br Mycol Soc 59:261–271

    Article  Google Scholar 

  • Ohtsuka N, Sou K, Amano T, Ojima M, Nakazawa Y, Yamada Y (1988) Decreased sensitivity of cucumber powdery mildew (Sphaerotheca fuliginea) to ergosterol biosynthesis inhibitors. Ann Phytopathol Soc Jpn 54:629–632

    Article  CAS  Google Scholar 

  • Olaya G, Cleere S, Stanger C, Burbidge J, Hall A, Windass J (2003) A novel potential target site QoI fungicide resistance mechanism in Pythium aphanidermatum (abstract). Phytopathology 93:S67

    Google Scholar 

  • Oostendorp M, Kunz W, Dietrich B, Staub T (2001) Induced disease resistance in plants by chemicals. Eur J Plant Pathol 107:19–28

    Article  CAS  Google Scholar 

  • Pasche JS, Wharam CM, Gudmestad NC (2004) Shift in sensitivity of Alternaria solani to QoI fungicides. Plant Dis 88:181–187

    Article  CAS  Google Scholar 

  • Ruess W, Müeller K, Knauf-Beiter G, Kunz W, Staub T (1996) Plant activator CGA245704: an innovative approach for disease control in cereals and tobacco. In: Proc. BCPC conf.—pests and diseases, BCPC, Alton, Hants, UK, pp 53–60

  • Sauter H, Steglich W, Anke T (1999) Strobilurine: evolution einer neuen wirkstoffklasse. Angew Chemie 111:1416–1438

    Article  Google Scholar 

  • Stadnik MJ, Buchenaueer H (2000) Inhibition of phenylalanine ammonia-lyase suppresses the resistance induced by benzothiadiazole in wheat to Blumeria graminis f. sp. tritici. Physiol Mol Plant Pathol 57:25–34

    Article  CAS  Google Scholar 

  • Tsubaki K, Tokumatsu S, Ando K, Nakagiri A, Okada G (1998) An illustrated guide to mitosporic fungi (deuteromycetes)-collection, isolation and identification (in Japanese). Industrial Publishing & Consulting, Tokyo, pp 48–53

  • Vallad GE, Goodman RM (2004) Systemic acquired resistance and induced systemic resistance in conventional agriculture. Crop Sci 44:1920–1934

    Article  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322

    Google Scholar 

  • Yamaguchi J, Inada M, Matsuzaki M (2000) Occurrence of DMI fungicide resistant isolates of Mycovellosiella nattrassii Deighton, causal fungus of leaf mold of eggplant (in Japanese). Jpn J Phytopathol 66:78–84

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki, 305-8604, Japan

    Wen-Hsin Chung, Saligrama Adavigowda Deepak & Hideo Ishii

  2. Department of Pant Pathology, National Chung Hsing University, 250, Kuo Kuang Rd., Taichung, 402, Taiwan

    Wen-Hsin Chung

  3. GANIT Labs IBAB, Biotech Park, Electronic City Phase I, Bangalore, 560 158, India

    Saligrama Adavigowda Deepak

Authors
  1. Wen-Hsin Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Saligrama Adavigowda Deepak
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hideo Ishii
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wen-Hsin Chung.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chung, WH., Deepak, S.A. & Ishii, H. Effect of acibenzolar-S-methyl, strobilurins and other fungicide treatments on phylloplane fungi of crops. J Gen Plant Pathol 79, 128–135 (2013). https://doi.org/10.1007/s10327-013-0433-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10327-013-0433-y

Keywords

Search

Navigation