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PCR–RFLP identifies differences in hrpZ sequences to distinguish two genetic groups of Pseudomonas syringae pv. syringae strains from barley and wheat with bacterial black nod

  • Bacterial and Phytoplasma Diseases
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Abstract

Differences in hrpZ sequences determined by polymerase chain reaction (PCR)–restriction fragment length polymorphism (RFLP) were used to investigate the molecular epidemiology of Pseudomonas syringae pv. syringae (PSS) strains that were isolated from diseased barley and wheat plants in Okayama, Japan. PCR–RFLP using HhaI separated PSS strains into two groups (A and B). Although specific PCR–RFLP groups of PSS strains were not always isolated from specific cultivars or seeds produced in a specific area, many strains isolated from barley and wheat belonged to PCR–RFLP groups A and B, respectively.

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Authors and Affiliations

  1. Research Institute for Agriculture, Okayama Prefectural Technology Center for Agriculture, Forestry and Fisheries, 1174-1 Koudaoki, Akaiwa City, Okayama, 709-0801, Japan

    Akira Kawaguchi

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  1. Akira Kawaguchi
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Correspondence to Akira Kawaguchi.

Additional information

The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accessions AB720061 and AB720062.

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Kawaguchi, A. PCR–RFLP identifies differences in hrpZ sequences to distinguish two genetic groups of Pseudomonas syringae pv. syringae strains from barley and wheat with bacterial black nod. J Gen Plant Pathol 79, 51–55 (2013). https://doi.org/10.1007/s10327-012-0420-8

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  • DOI: https://doi.org/10.1007/s10327-012-0420-8

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