Abstract
We determined the complete nucleotide sequence of RNA-1 and the 5″-terminal region of RNA-2 from Broad bean wilt virus 1 (BBWV-1) isolate PV132. This report is the first analysis of the genome organization of BBWV-1. We also determined the complete nucleotide sequence of RNA-1 from Broad bean wilt virus 2 (BBWV-2) isolate IP and analyzed the genetic relations between BBWV-1 and BBWV-2. Similar to the BBWV-2 isolates, both RNAs of PV132 encoded a single large polyprotein, which was predicted to contain some functional proteins in a manner similar to those of comovirus. With respect to the deduced amino acid sequences of the mature proteins, PV132 and IP had only 20%–40% homology to comovirus. On the other hand, IP was 73%–98% homologous to BBWV-2 isolates, but PV132 was 39%–67% homologous to the isolates. Although the extent of the homologies differed, the homologies were limited between BBWV-1 and BBWV-2 not only for the coat protein but also for the other proteins. These results clearly support the placement of BBWV-1 and BBWV-2 in the genus Fabavirus as distinct species, proposed on the basis of double immunodiffusion tests.
Similar content being viewed by others
Author information
Authors and Affiliations
Corresponding author
Additional information
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession numbers AB084450 (RNA-1 of isolate PV132), AB084451 (RNA-2 of isolate PV132), and AB023484 (RNA-1 of isolate IP)
Rights and permissions
About this article
Cite this article
Kobayashi, Y., Kobayashi, A., Nakano, M. et al. Analysis of genetic relations between Broad bean wilt virus 1 and Broad bean wilt virus 2 . J Gen Plant Pathol 69, 320–326 (2003). https://doi.org/10.1007/s10327-003-0053-z
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10327-003-0053-z