Original Paper

Environmental Chemistry Letters

, Volume 4, Issue 2, pp 69-73

Nitrate reductase for nitrate analysis in water

  • Wilbur H. CampbellAffiliated withThe Nitrate Elimination Co., Inc.Biological Sciences Department, Michigan Technological University Email author 
  • , Pengfei SongAffiliated withBiological Sciences Department, Michigan Technological University
  • , Guillaume G. BarbierAffiliated withThe Nitrate Elimination Co., Inc.Biological Sciences Department, Michigan Technological University

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Abstract

Nitrate analysis in water is one of the most frequently applied methods in environmental chemistry. Current methods for nitrate are generally based on toxic substances. Here, we show that a viable alternative method is to use the enzyme nitrate reductase. The key to applying this Green Chemistry solution for nitrate analysis is plentiful, inexpensive, analytical grade enzyme. We demonstrate that recombinant Arabidopsis nitrate reductase, expressed in the methylotrophic yeast Pichia pastoris, is a highly effective catalyst for nitrate analysis at 37°C. Recombinant production of enzyme ensures consistent quality and provides means to meet the needs of environmental chemistry.

Keywords

Nitrate reductase Recombinant expression Nitrate Green chemistry Water Enzymes