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Bacterial community shift for monitoring the co-composting of oil palm empty fruit bunch and palm oil mill effluent anaerobic sludge

  • Environmental Microbiology - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

A recently developed rapid co-composting of oil palm empty fruit bunch (OPEFB) and palm oil mill effluent (POME) anaerobic sludge is beginning to attract attention from the palm oil industry in managing the disposal of these wastes. However, a deeper understanding of microbial diversity is required for the sustainable practice of the co-compositing process. In this study, an in-depth assessment of bacterial community succession at different stages of the pilot scale co-composting of OPEFB-POME anaerobic sludge was performed using 454-pyrosequencing, which was then correlated with the changes of physicochemical properties including temperature, oxygen level and moisture content. Approximately 58,122 of 16S rRNA gene amplicons with more than 500 operational taxonomy units (OTUs) were obtained. Alpha diversity and principal component analysis (PCoA) indicated that bacterial diversity and distributions were most influenced by the physicochemical properties of the co-composting stages, which showed remarkable shifts of dominant species throughout the process. Species related to Devosia yakushimensis and Desemzia incerta are shown to emerge as dominant bacteria in the thermophilic stage, while Planococcus rifietoensis correlated best with the later stage of co-composting. This study proved the bacterial community shifts in the co-composting stages corresponded with the changes of the physicochemical properties, and may, therefore, be useful in monitoring the progress of co-composting and compost maturity.

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Acknowledgements

The authors would like to thank Kyushu Institute of Technology and Kyushu University for funding, equipment and technical supports for this research and also Universiti Putra Malaysia for providing a Ph.D. fellowship.

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Authors and Affiliations

  1. Laboratory of Sustainable Animal Production and Biodiversity, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    Mohd Huzairi Mohd Zainudin

  2. Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    Norhayati Ramli & Mohd Ali Hassan

  3. Department of Biological Function and Engineering, Graduate School of Life Science and System Engineering, Kyushu Institute of Technology, 2-4 Hibikino-cho, Wakamatsu-Ku, Fukuoka, 808-0196, Japan

    Yoshihito Shirai

  4. Laboratory of Molecular Gene Technique, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-Ku, Fukuoka, 812-8581, Japan

    Kosuke Tashiro

  5. Laboratory of Soil Microbiology, Department of Bioscience and Biotechnology, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, 6-10-1 Higashi-Ku, Fukuoka, 812-8581, Japan

    Kenji Sakai & Yukihiro Tashiro

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  1. Mohd Huzairi Mohd Zainudin
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Correspondence to Norhayati Ramli.

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Zainudin, M.H.M., Ramli, N., Hassan, M.A. et al. Bacterial community shift for monitoring the co-composting of oil palm empty fruit bunch and palm oil mill effluent anaerobic sludge. J Ind Microbiol Biotechnol 44, 869–877 (2017). https://doi.org/10.1007/s10295-017-1916-1

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  • DOI: https://doi.org/10.1007/s10295-017-1916-1

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