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Endophytic Streptomyces sp. AC35, a producer of bioactive isoflavone aglycones and antimycins

  • Natural Products
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

In this research, a microbial endophytic strain obtained from the rhizosphere of the conifer Taxus baccata and designated as Streptomyces sp. AC35 (FJ001754.1 Streptomyces, GenBank) was investigated. High 16S rDNA gene sequence similarity suggests that this strain is closely related to S. odorifer. The major fatty acid profile of intracellular lipids was also carried out to further identify this strain. Atomic force microscopy and scanning acoustic microscopy were used to image our strain. Its major excreted substances were extracted, evaluated for antimicrobial activity, purified, and identified by ultraviolet–visible spectroscopy (UV–vis), liquid chromatography–mass spectrometry (LC–MS/MS) and nuclear magnetic resonance as the bioactive isoflavone aglycones—daidzein, glycitein and genistein. Batch cultivation, performed under different pH conditions, revealed enhanced production of antimycin components when the pH was stable at 7.0. Antimycins were detected by HPLC and identified by UV–vis and LC–MS/MS combined with the multiple reaction monitoring. Our results demonstrate that Streptomyces sp. AC35 might be used as a potential source of effective, pharmaceutically active compounds.

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Authors and Affiliations

  1. Institute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Bratislava, Slovakia

    P. Ondrejíčková, M. Šturdíková, K. Markošová & M. Čertík

  2. Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Bratislava, Slovakia

    A. Hushegyi

  3. Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic

    E. Švajdlenka

Authors
  1. P. Ondrejíčková
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  2. M. Šturdíková
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  3. A. Hushegyi
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  4. E. Švajdlenka
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  5. K. Markošová
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  6. M. Čertík
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Correspondence to P. Ondrejíčková.

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Funding

This study was supported by VEGA Grants No. 1/0770/15 from Ministry of Education, Slovak Republic.

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The authors declare that they have no conflict of interest.

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Ondrejíčková, P., Šturdíková, M., Hushegyi, A. et al. Endophytic Streptomyces sp. AC35, a producer of bioactive isoflavone aglycones and antimycins. J Ind Microbiol Biotechnol 43, 1333–1344 (2016). https://doi.org/10.1007/s10295-016-1800-4

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  • DOI: https://doi.org/10.1007/s10295-016-1800-4

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