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Recycling of lipid-extracted hydrolysate as nitrogen supplementation for production of thraustochytrid biomass

  • Fermentation, Cell Culture and Bioengineering
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Efficient resource usage is important for cost-effective microalgae production, where the incorporation of waste streams and recycled water into the process has great potential. This study builds upon emerging research on nutrient recycling in thraustochytrid production, where waste streams are recovered after lipid extraction and recycled into future cultures. This research investigates the nitrogen flux of recycled hydrolysate derived from enzymatic lipid extraction of thraustochytrid biomass. Results indicated the proteinaceous content of the recycled hydrolysate can offset the need to supply fresh nitrogen in a secondary culture, without detrimental impact upon the produced biomass. The treatment employing the recycled hydrolysate with no nitrogen addition accumulated 14.86 g L−1 of biomass in 141 h with 43.3 % (w/w) lipid content compared to the control which had 9.26 g L−1 and 46.9 % (w/w), respectively. This improved nutrient efficiency and wastewater recovery represents considerable potential for enhanced resource efficiency of commercial thraustochytrid production.

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Acknowledgments

This work was supported by Mitacs through the Mitacs-Accelerate Program (IT04538) in partnership with Dalhousie University and Mara Renewables Corporation and support from the Natural Sciences and Engineering Research Council (NSERC) of Canada. Special thanks for technical support owed to Analytical Chemist Spencer Scott and the staff at Mara Renewables Corporation.

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Authors and Affiliations

  1. Department of Process Engineering and Applied Science, Faculty of Engineering, Dalhousie University, Halifax, NS, B3H 4R2, Canada

    Joshua Lowrey, Roberto E. Armenta & Marianne S. Brooks

  2. Mara Renewables Corporation, 101 Research Drive, Dartmouth, NS, B2Y 4T6, Canada

    Joshua Lowrey & Roberto E. Armenta

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  1. Joshua Lowrey
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  2. Roberto E. Armenta
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Correspondence to Joshua Lowrey.

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Funding

This work was supported by Mitacs through the Mitacs-Accelerate Program (IT04538) in partnership with Dalhousie University and Mara Renewables Corporation.

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Joshua Lowrey declares that he has no conflict of interest. Roberto E. Armenta declares that she has no conflict of interest. Marianne S. Brooks declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Lowrey, J., Armenta, R.E. & Brooks, M.S. Recycling of lipid-extracted hydrolysate as nitrogen supplementation for production of thraustochytrid biomass. J Ind Microbiol Biotechnol 43, 1105–1115 (2016). https://doi.org/10.1007/s10295-016-1779-x

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