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Streptomyces sanglieri which colonised and enhanced the growth of Elaeis guineensis Jacq. seedlings was antagonistic to Ganoderma boninense in in vitro studies

  • Environmental Microbiology
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Actinomycete strain AUM 00500 was 99.5 % similar to Streptomyces sanglieri NBRC 100784T and was evaluated for antagonistic activity towards Ganoderma boninense, the causative fungus of basal stem rot of oil palm. The strain showed strong antifungal activity towards G. boninense in in vitro and SEM analysis showed various modes of inhibition of the fungus. Ethyl acetate extracts of single culture and inhibition zone of cross-plug culture by HPLC indicated that strain AUM 00500 produced two different antibiotics of the glutarimide group namely cycloheximide and actiphenol. In greenhouse trials, oil palm seed treated with spores of S. sanglieri strain AUM 00500 at 109 cfu/ml showed significant (P < 0.05) increase in oil palm seedlings growth when compared to the control. Streptomyces sanglieri strain AUM 00500 successfully colonised the epidermal surface of the roots of treated oil palm seedlings and it was recovered from root fragments plated on starch casein agar.

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Acknowledgments

The authors would like to thank the University of Malaya for Grants (PS189/2008B) and (J-21001-76536). The authors also thank the Applied Agricultural Resources Sdn. Bhd. (for the seeds) and Sime Darby Plantation Sdn. Bhd. (for Ganoderma boninense culture) used in this study. Mr. Roslee Halip is thanked for his assistance with SEM.

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Authors and Affiliations

  1. Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia

    A. B. Nur Azura, M. Yusoff, G. Y. A. Tan & S. Vikineswary

  2. Mushroom Research Centre, University of Malaya, 50603, Kuala Lumpur, Malaysia

    A. B. Nur Azura, G. Y. A. Tan, R. Jegadeesh & S. Vikineswary

  3. Sime Darby Technology Centre Sdn. Bhd., 1st Floor, Block B, UPM-MTDC Technology Centre III, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    D. R. Appleton

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  1. A. B. Nur Azura
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Correspondence to S. Vikineswary.

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Nur Azura, A.B., Yusoff, M., Tan, G.Y.A. et al. Streptomyces sanglieri which colonised and enhanced the growth of Elaeis guineensis Jacq. seedlings was antagonistic to Ganoderma boninense in in vitro studies. J Ind Microbiol Biotechnol 43, 485–493 (2016). https://doi.org/10.1007/s10295-015-1724-4

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