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Immobilization of Pseudomonas fluorescens lipase on hydrophobic supports and application in biodiesel synthesis by transesterification of vegetable oils in solvent-free systems

  • Biocatalysis
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

This work describes the preparation of biocatalysts for ethanolysis of soybean and babassu oils in solvent-free systems. Polystyrene, Amberlite (XAD-7HP), and octyl-silica were tested as supports for the immobilization of Pseudomonas fluorescens lipase (PFL). The use of octyl-silica resulted in a biocatalyst with high values of hydrolytic activity (650.0 ± 15.5 IU/g), immobilization yield (91.3 ± 0.3 %), and recovered activity (82.1 ± 1.5 %). PFL immobilized on octyl-silica was around 12-fold more stable than soluble PFL, at 45 °C and pH 8.0, in the presence of ethanol at 36 % (v/v). The biocatalyst provided high vegetable oil transesterification yields of around 97.5 % after 24 h of reaction using babassu oil and around 80 % after 48 h of reaction using soybean oil. The PFL-octyl-silica biocatalyst retained around 90 % of its initial activity after five cycles of transesterification of soybean oil. Octyl-silica is a promising support that can be used to immobilize PFL for subsequent application in biodiesel synthesis.

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Acknowledgments

The authors are grateful for the financial support provided by the Brazilian research foundations CNPq, FAPESP, and CAPES. The authors also thank the Catalysis Laboratory of the Department of Chemical Engineering of the Federal University of São Carlos for the thermogravimetric and surface area analyses, and the Postgraduate Program in Chemical Engineering of the Federal University of São Carlos (PPGEQ/UFSCar), where this work was undertaken.

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Authors and Affiliations

  1. Graduate Program in Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, São Carlos, SP, 13565-905, Brazil

    Lionete N. Lima, Gladson C. Oliveira, Mayerlenis J. Rojas, Raquel L. C. Giordano & Paulo W. Tardioli

  2. Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, Lorena, Brazil

    Heizir F. Castro & Patrícia C. M. Da Rós

  3. Institute of Chemistry, Federal University of Alfenas, Alfenas, Brazil

    Adriano A. Mendes

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  1. Lionete N. Lima
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Lima, L.N., Oliveira, G.C., Rojas, M.J. et al. Immobilization of Pseudomonas fluorescens lipase on hydrophobic supports and application in biodiesel synthesis by transesterification of vegetable oils in solvent-free systems. J Ind Microbiol Biotechnol 42, 523–535 (2015). https://doi.org/10.1007/s10295-015-1586-9

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