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Direct starch fermentation to l-lactic acid by a newly isolated thermophilic strain, Bacillus sp. MC-07

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Journal of Industrial Microbiology & Biotechnology

Abstract

A newly isolated Bacillus sp. MC-07 showed 99.2 % 16S rRNA gene sequence similarity with the Bacillus thermoamylovorans LMG 18084T. It demonstrated optimum and maximum growth temperatures of 50 and 62 °C, respectively. The ability of MC-07 to produce optically pure l-lactic acid via direct fermentation of starch without enzymatic hydrolysis was investigated at different pH values (6.0–8.0) by intermittent adjustments every 12 h. During batch fermentation in mineral salt medium containing 0.001 % yeast extract at pH 7.0, 20 g/L of soluble starch was utilized to produce 16.6 g/L l-lactic acid at 50 °C within 24 h of fermentation, with 100 % optical purity, 92.1 % lactic acid selectivity, and an l-lactic acid yield of 0.977 g/g. Direct starch fermentation at pHs 6.0, 6.5, 7.5, and 8.0 resulted in considerably lower concentrations of lactic acid than did at pH 7.0. Compared with B. thermoamylovorans LMG 18084T, the ability of strain MC-07 to produce l-lactic acid was superior.

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Acknowledgments

This work was supported partly by JSPS KAKENHI Grant number 26740050 and by Kyushu University interdisciplinary programs in education and projects in research development.

Conflict of interest

We have no conflict of interest to declare.

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Authors and Affiliations

  1. Laboratory of Soil Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Pramod Poudel, Yukihiro Tashiro, Yuki Okugawa & Kenji Sakai

  2. Institute of Advanced Study, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Yukihiro Tashiro

  3. Japan Eco-Science (Nikkan Kagaku) Co. Ltd, 11-1-2F Shiomigaokacho, Chuo-ku, Chiba, 260-0034, Japan

    Hirokuni Miyamoto

  4. Graduate School of Advanced Integration Science, Chiba University, 1-33 Yayoi-cho, Chiba, 263-8522, Japan

    Hirokuni Miyamoto

  5. Department of Biochemistry and Integrative Medical Biology, Keio School of Medicine, Shinanomachi 35, Shinjuku-ku, Tokyo, 160-8582, Japan

    Hirokuni Miyamoto

  6. Sermas Co. Ltd, Ichikawa minami 2-8-8, Ichikawa, Chiba, 272-0033, Japan

    Hirokuni Miyamoto & Hisashi Miyamoto

  7. Miroku Co. Ltd, Iwaya 706-27, Kitsuki, Oita, 873-0006, Japan

    Hisashi Miyamoto

Authors
  1. Pramod Poudel
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  2. Yukihiro Tashiro
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  3. Hirokuni Miyamoto
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  4. Hisashi Miyamoto
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  5. Yuki Okugawa
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  6. Kenji Sakai
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Corresponding author

Correspondence to Kenji Sakai.

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Poudel, P., Tashiro, Y., Miyamoto, H. et al. Direct starch fermentation to l-lactic acid by a newly isolated thermophilic strain, Bacillus sp. MC-07. J Ind Microbiol Biotechnol 42, 143–149 (2015). https://doi.org/10.1007/s10295-014-1534-0

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  • DOI: https://doi.org/10.1007/s10295-014-1534-0

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