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Preparation of przewalskinic acid A from salvianolic acid B using a crude enzyme from an Aspergillus oryzae strain

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Journal of Industrial Microbiology & Biotechnology

Abstract

Przewalskinic acid A is a rare, water-soluble, and highly biologically active ingredient found, thus far, only in the Salvia przewalskii Maxim herb; however, the content in S. przewalskii herb is very low. In order to obtain useful quantities of przewalskinic acid A, the biotransformatin of salvianolic acid B from Salvia miltiorrhiza root (danshen in Chinese) into przewalskinic acid A was studied using a crude enzyme produced from Aspergillus oryzae D30s strain. The crude enzyme from the A. oryzae strain hydrolyzed salvianolic acid B into przewalskinic acid A and danshensu. The preparation afforded 31.3 g przewalskinic acid A (91.0 % purity) and 13.1 g danshensu (95 % purity) from 75 g salvianolic acid B. The preparation of przewalskinic acid A was therefore very successful with a yield of over 86 %, but the yield of danshensu was only 33 %. The product przewalskinic acid A was identified using ultra-performance liquid chromatography–mass spectrometry (UPLC–MS) and NMR.

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Acknowledgments

This work was supported by the Major Projects of National Science and Technology “Significant new drugs creation” Project of China No. 2012ZX09503001-003; the Program for Liaoning Innovative Research Teams in Universities (LNIRT: 2009T007, LT2010009) and the National Science of Foundation of the People’s Republic of China (NSFC).

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Authors and Affiliations

  1. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Science, Jilin University, Changchun, 130012, People’s Republic of China

    Chunying Liu & Yinghua Jin

  2. College of Biotechnology, Dalian Polytechnic University, Dalian, 116034, People’s Republic of China

    Chunying Liu, Hongshan Yu, Longquan Xu, Chong Yu & Fengxie Jin

  3. Institute for Brain Disorders, Dalian Medical University, Dalian, 116044, People’s Republic of China

    Changkai Sun

  4. Tianjin Tasly Institute, Tianjin, 300402, People’s Republic of China

    Shuiping Zhou & Yonghong Zhu

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  1. Chunying Liu
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  2. Yinghua Jin
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  3. Hongshan Yu
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  4. Changkai Sun
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  5. Shuiping Zhou
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  6. Yonghong Zhu
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  7. Longquan Xu
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  8. Chong Yu
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  9. Fengxie Jin
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Correspondence to Hongshan Yu, Changkai Sun or Fengxie Jin.

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Liu, C., Jin, Y., Yu, H. et al. Preparation of przewalskinic acid A from salvianolic acid B using a crude enzyme from an Aspergillus oryzae strain. J Ind Microbiol Biotechnol 41, 887–892 (2014). https://doi.org/10.1007/s10295-013-1399-7

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  • DOI: https://doi.org/10.1007/s10295-013-1399-7

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