Bioenergy/Biofuels/Biochemicals

Journal of Industrial Microbiology & Biotechnology

, Volume 40, Issue 2, pp 227-233

The role of aldehyde/alcohol dehydrogenase (AdhE) in ethanol production from glycerol by Klebsiella pneumoniae

  • Baek-Rock OhAffiliated withApplied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB)
  • , Won-Kyung HongAffiliated withApplied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB)
  • , Sun-Yeon HeoAffiliated withApplied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB)
  • , Min-ho JoeAffiliated withRadiation Research Division for Biotechnology, Korea Atomic Energy Research Institute
  • , Jeong-Woo SeoAffiliated withApplied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB) Email author 
  • , Chul Ho KimAffiliated withApplied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB) Email author 

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Abstract

Transcriptome analysis of a K. pneumoniae GEM167 mutant strain derived by irradiation with gamma rays, which exhibited high-level production of ethanol from glycerol, showed that the mutant expressed AdhE at a high level. Ethanol production decreased significantly, from 8.8 to 0.5 g l−1, when an adhE-deficient derivative of that strain was grown on glycerol. Bacterial growth was also reduced under such conditions, showing that AdhE plays a critical role in maintenance of redox balance by catalyzing ethanol production. Overexpression of AdhE enhanced ethanol production, from pure or crude glycerol, to a maximal level of 31.9 g l−1 under fed-batch fermentation conditions; this is the highest level of ethanol production from glycerol reported to date.

Keywords

Klebsiella pneumoniae Glycerol Ethanol Aldehyde/alcohol dehydrogenase (AdhE)