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Characterization of a new alginate lyase from newly isolated Flavobacterium sp. S20

  • Fermentation, Cell Culture and Bioengineering
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Alginate lyase is a promising biocatalyst because of its application in saccharification of alginate for the production of biochemicals and renewable biofuels. This study described the isolation of a new alginate metabolizing bacterium, Flavobacterium sp. S20, from sludge samples and the characterization of its alginate lyase Alg2A. The alginate lyase gene, alg2A, was obtained by constructing and screening the genomic library of the strain S20 and overexpressed in Escherichia coli. Substrate specificity assays indicated Alg2A preferred poly-α-l-guluronate as a substrate over poly-β-d-mannuronate. In the saccharification process of a high content (10 %, w/v) of sodium alginate, the recombinant alginate lyase Alg2A yielded 152 of mM the reducing sugars after 69 h of reaction, and the amounts of oligosaccharides with a different degree of polymerization (DP) generated by Alg2A gradually accumulated without significant variation in the distribution of oligosaccharide compositions. These results indicated that Alg2A possessed high enzymatic capability for saccharifying the alginate, which could be used in saccharifying the alginate biomass prior to the main fermentation process for biofuels. In addition, Alg2A had a different endolytic reaction mode from both the two commercial alginate lyases and other alginate lyases from polysaccharide lyase family 7 owing to high yields of penta-, hex-, and hepta-saccharides in the hydrolysis products of Alg2A. Thus, Alg2A could be a good tool for the large-scale preparation of alginate oligosaccharides with high DP.

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Acknowledgments

This work was granted by Chinese High-tech Research and Development program (2011AA090704 and 2012AA021501), the National Department Public Benefit Research Foundation of China (200903052), and the Liaoning Doctoral Startup Project (20111150). We thank Dr. Bo Shi, Feed Research Institute Chinese Academy of Agricultural Sciences, for his technical guidance in preparation of alginate block structures. We also thank Dr. Zhongfu Wang and Dr. Linjuan Huang, Northwest University, for their guidance of analyzing the ESI–MS data.

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Authors and Affiliations

  1. Natural Products and Glyco-Biotechnology Research Group, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, CAS, Dalian, 116023, People’s Republic of China

    Lishuxin Huang, Qiang Peng & Yuguang Du

  2. State Key Laboratory of Genetic Engineering, Fudan University, Shanghai, 200433, People’s Republic of China

    Jungang Zhou & Hong Lu

  3. Shan Xi Provincial Key Laboratory of Biotechnology, Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Life Science College, Northwest University, Xi’an, 710069, People’s Republic of China

    Xiao Li

  4. Graduate University of the Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Lishuxin Huang & Qiang Peng

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  1. Lishuxin Huang
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  2. Jungang Zhou
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  3. Xiao Li
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  4. Qiang Peng
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  5. Hong Lu
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Correspondence to Jungang Zhou or Yuguang Du.

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Huang, L., Zhou, J., Li, X. et al. Characterization of a new alginate lyase from newly isolated Flavobacterium sp. S20. J Ind Microbiol Biotechnol 40, 113–122 (2013). https://doi.org/10.1007/s10295-012-1210-1

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  • DOI: https://doi.org/10.1007/s10295-012-1210-1

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