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Microwave radiation and reactor design influence microbial communities during methane fermentation

  • Genetics and Molecular Biology of Industrial Organisms
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The effect of reactor design and method of heating on the efficiency of methane fermentation and composition of microbial communities, especially methanogenic Archaea, were determined. The research was carried out using submerge- and trickling-bed reactors fed with wastewater and the heat supply into the reactors included a convection heating method and microwave radiation. The polymerase chain reaction-denaturing gradient gel electrophoresis and relative real-time PCR were used in order to assess the biofilm communities. The best fermentation results and the highest abundance of methanogenic Archaea in biomass were observed in microwave heated trickling-bed reactors. The research proved that in reactors of identical design, the application of microwaves enabled a higher fermentation efficiency to be obtained and simultaneously increased the diversity of methanogenic Archaea communities that favors process stability. All the identified sequences of Archaea belonged to Methanosarcina sp., suggesting that species from this genera are susceptible to non-thermal effects of microwaves. There were no effects from microwaves on the bacterial communities in both types of reactors, however, the bacterial species composition varied in the reactors of different design.

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Acknowledgments

We are grateful for the financial support of the Ministry of Science and Higher Education Republic of Poland (Project No. N N523 455436). All authors have agreed to submit the manuscript to ‘Journal of Industrial Microbiology and Biotechnology’.

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Authors and Affiliations

  1. Department of Environmental Biotechnology, Faculty of Environmental Sciences and Fisheries, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

    Agnieszka Cydzik-Kwiatkowska & Paulina Jaranowska

  2. Department of Environment Protection Engineering, Faculty of Environmental Sciences and Fisheries, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

    Marcin Zieliński

Authors
  1. Agnieszka Cydzik-Kwiatkowska
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  2. Marcin Zieliński
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  3. Paulina Jaranowska
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Correspondence to Agnieszka Cydzik-Kwiatkowska.

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Cydzik-Kwiatkowska, A., Zieliński, M. & Jaranowska, P. Microwave radiation and reactor design influence microbial communities during methane fermentation. J Ind Microbiol Biotechnol 39, 1397–1405 (2012). https://doi.org/10.1007/s10295-012-1141-x

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  • DOI: https://doi.org/10.1007/s10295-012-1141-x

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