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Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro

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Abstract

The aim of this study was to assess the factors, mechanisms and the differences between periodontal ligament (PDL) cells and denta l follicle (DF) progenitor cells towards the osteoblastic/cementoblastic differentiation and to investigate the effects of BMP-7 on developmental (DF) and mature tissue-derived (PDL) cells, respectively. Primary cell culture of PDL cells and DF progenitor cells was performed. Osteogenic differentiation was evaluated using von Kossa, Alizarin Red S and immuno-histo-chemistry staining of osteocalcin. Gene expression pattern was evaluated via real-time PCR. A series of CD surface marks were tested using flow cytometry and fluorescence-activated cell-sorting analysis was performed. Real-time RT-PCR demonstrated similar gene expression pattern of PDL cells and DF progenitor cells: the expression of OPN and OCN significantly was elevated when incubated with osteogenic components, Runx2 was unaffected, and Osteorix was hardly expressed whether in basic medium or induction medium. In addition, BMP-7 induced osteoblast/cementoblast differentiation of PDLSCs and DF progenitor cells in a dose- and time-dependent manner, as reflected by enhanced Runx2 and (OCN) mRNA transcript expression. BMP-7 triggers PDL cells and DF progenitor cells to differentiate towards an osteoblast/cementoblast phenotype.

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Acknowledgments

The authors would like to thank Prof. Dr. Cengiz Han Acikel from Department of Biostatistics at Gülhane Military Medical Academy for his special effort in conducting the statistical analysis of the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Christian Albrechts University, Kiel, Germany

    Yahya Açil, Jörg Wiltfang & Matthias Gierloff

  2. Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, China

    Fan Yang

  3. Centre for Oral Health and Dental Management, Mevki Military Hospital, 06130, Diskapi Altindag, Ankara, Turkey

    Aydin Gulses

  4. Private practice, Duisburg, Germany

    Mustafa Ayna

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  1. Yahya Açil
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  2. Fan Yang
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Correspondence to Aydin Gulses.

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Açil, Y., Yang, F., Gulses, A. et al. Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro. Odontology 104, 123–135 (2016). https://doi.org/10.1007/s10266-015-0198-1

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