Journal of Plant Research

, Volume 129, Issue 5, pp 921–933

Heterologous expression of the Hsp24 from Trichoderma asperellum improves antifungal ability of Populus transformant Pdpap-Hsp24 s to Cytospora chrysosperma and Alternaria alternate

  • S. D. Ji
  • Z. Y. Wang
  • H. J. Fan
  • R. S. Zhang
  • Z. Y. Yu
  • J. J. Wang
  • Z. H. Liu
Regular Paper

DOI: 10.1007/s10265-016-0829-9

Cite this article as:
Ji, S.D., Wang, Z.Y., Fan, H.J. et al. J Plant Res (2016) 129: 921. doi:10.1007/s10265-016-0829-9

Abstract

The tolerance of plants to biotic and abiotic stresses could be improved by transforming with fungal resistance-related genes. In this study, the cDNA sequence (GenBank Acc. No. KP337939) of the resistance-related gene Hsp24 encoding the 24 kD heat shock protein was obtained from the biocontrol fungus Trichoderma asperellum ACCC30536. The promoter region of Hsp24 contained many cis-regulators related to stresses response, such as “GCN4” and “GCR1” etc. Hsp24 transcription in T. asperellum was up-regulated under six different environmental stresses, compared with the control. Furthermore, following heterologous transformation into Populus davidiana × P. alba var. Pyramidalis (Pdpap), Hsp24 was successfully transcribed in transformant Pdpap-Hsp24s. Pathogen-related genes (PRs) in four Pdpap-Hsp24s were up-regulated compared with those in the control Pdpap (Pdpap-Con). After co-culture of Pdpap-Hsp24s with the weak parasite Cytospora chrysosperma, the transcription of genes related to hormone signal pathway (JA and SA) were up-regulated in Pdpap-Hsp24s, and ethidium bromide (EtBr) and Nitro-blue tetrazolium (NBT) staining assays indicated that the cell membrane permeability and the active oxygen content of Pdpap-Hsp24s leaves were lower than that of the control Pdpap-Con. And when the Pdpap-Hsp24s were under the Alternaria alternate stress, the activities of superoxide dismutase (SOD) and peroxidase (POD) got higher in Pdpap-Hsp24s than that in Pdpap-Con, and the disease spots in Pdpap-Con leaves were obviously larger than those in Pdpap-Hsp24s leaves. In summary, Hsp24 of T. asperellum ACCC30536 is an important defense response gene, and its heterologous expression improved the resistance of transformant Pdpap-Hsp24s to C. chrysosperma and A. alternate.

Keywords

Populus Trichoderma asperellum Heat shock protein Plant transgene Antifungus 

Supplementary material

10265_2016_829_MOESM1_ESM.doc (69 kb)
Supplementary material 1 (DOC 69 kb)

Copyright information

© The Botanical Society of Japan and Springer Japan 2016

Authors and Affiliations

  • S. D. Ji
    • 1
  • Z. Y. Wang
    • 1
  • H. J. Fan
    • 1
  • R. S. Zhang
    • 2
  • Z. Y. Yu
    • 1
  • J. J. Wang
    • 1
  • Z. H. Liu
    • 1
  1. 1.School of ForestryNortheast Forestry UniversityHarbinChina
  2. 2.The College of LandscapeNortheast Forestry UniversityHarbinChina