Journal of Plant Research

, Volume 129, Issue 5, pp 899–907

A pqr2 mutant encodes a defective polyamine transporter and is negatively affected by ABA for paraquat resistance in Arabidopsis thaliana

  • Shuchao Dong
  • Huizhen Hu
  • Youmei Wang
  • Zhengdan Xu
  • Yi Zha
  • Xiwen Cai
  • Liangcai Peng
  • Shengqiu Feng
Regular Paper

DOI: 10.1007/s10265-016-0819-y

Cite this article as:
Dong, S., Hu, H., Wang, Y. et al. J Plant Res (2016) 129: 899. doi:10.1007/s10265-016-0819-y
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Abstract

Despite the paraquat-resistant mutants that have been reported in plants, this study identified a novel A. thaliana mutant (pqr2) from an XVE inducible activation library based on its resistance to 2 μM paraquat. The pqr2 mutant exhibited a termination mutation in the exon of AT1G31830/PAR1/PQR2, encoded a polyamine uptake transporter AtPUT2/PAR1/PQR2. The PQR2 mutation could largely reduce superoxide accumulation and cell death in the pqr2 plants under paraquat treatment. Moreover, compared with wild type, the pqr2 mutant exhibited much reduced tolerance to putrescine, a classic polyamine compound, which confirmed that PQR2 encoded a defective polyamine transporter. Notably, co-treated with ABA and paraquat, both pqr2 mutant and wild type exhibited a lethal phenotype from seed germination, but the wild type like pqr2 mutant, could remain paraquat-resistance while co-treated with high dosage of Na2WO4, an ABA synthesis inhibitor. Gene expression analysis suggested that ABA signaling should widely regulate paraquat-responsive genes distinctively in wild type and pqr2 mutant. Hence, this study has for the first time reported about ABA negative effect on paraquat-resistance in A. thaliana, providing insight into the ABA signaling involved in the oxidative stress responses induced by paraquat in plants.

Keywords

Polyamine transporter Paraquat resistance ABA A. thaliana 

Supplementary material

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Supplementary material 1 (TIFF 14899 kb)
10265_2016_819_MOESM2_ESM.doc (36 kb)
Supplementary material 2 (DOC 35 kb)
10265_2016_819_MOESM3_ESM.doc (42 kb)
Supplementary material 3 (DOC 42 kb)
10265_2016_819_MOESM4_ESM.doc (46 kb)
Supplementary material 4 (DOC 46 kb)

Copyright information

© The Botanical Society of Japan and Springer Japan 2016

Authors and Affiliations

  • Shuchao Dong
    • 1
    • 2
  • Huizhen Hu
    • 1
    • 2
  • Youmei Wang
    • 1
    • 2
  • Zhengdan Xu
    • 1
    • 2
  • Yi Zha
    • 1
    • 2
  • Xiwen Cai
    • 1
    • 3
  • Liangcai Peng
    • 1
  • Shengqiu Feng
    • 1
    • 2
  1. 1.Biomass and Bioenergy Research Centre, College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina
  2. 2.National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanChina
  3. 3.Department of Plant SciencesNorth Dakota State UniversityFargoUSA