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An inverse relationship between allelopathic activity and salt tolerance in suspension cultures of three mangrove species, Sonneratia alba, S. caseolaris and S. ovata: development of a bioassay method for allelopathy, the protoplast co-culture method

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Abstract

A bioassay method for allelopathy, the ‘protoplast co-culture method’ was developed to study the relationship between salt tolerance and allelopathy of three mangrove species, Sonneratia alba, S. caseolaris, and S. ovata. Plants of S. alba grow in the seaward-side high salinity region and plants of the latter two species grow in upstream-side regions of a mangrove forest, respectively. Effects of five sea salts (NaCl, KCl, MgCl2, MgSO4 and CaCl2) on the growth of the suspension cells of the latter two species were first investigated by a small-scale method using 24-well culture plates. S. ovata cells showed higher tolerance than S. caseolaris cells to NaCl and other salts, but were not as halophilic as S. alba cells. Protoplasts isolated from suspension cells were co-cultured with lettuce protoplasts in Murashige and Skoog’s (MS) basal medium containing 1 μM 2,4-dichlorophenoxyacetic acid, 0.1 μM benzyladenine, 3 % sucrose and 0.6–0.8 M osmoticum. S. caseolaris protoplasts had a higher inhibitory effect on lettuce protoplast cell divisions than S. alba protoplasts at any lettuce protoplast density, and the effect of S. ovata was intermediate between the two. These results were similar to those obtained from a different in vitro bioassay method for allelopathy, the ‘sandwich method’ with dried leaves. The inverse relationship between allelopathic activity and salt tolerance in suspension cells of Sonneratia mangroves is discussed.

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Acknowledgments

The authors thank Prof. E. C. Yeung of the University of Calgary for his valuable suggestions on the manuscript.

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Authors and Affiliations

  1. Graduate School of Environment and Information Sciences, Yokohama National University, Yokohama, 240-8501, Japan

    Ai Hasegawa & Tomoya Oyanagi

  2. Tokyo University of Agriculture, Tokyo, 156-8502, Japan

    Reiko Minagawa

  3. Tokyo University of Agriculture and Technology, Tokyo, 183-8509, Japan

    Yoshiharu Fujii

  4. Faculty of Environment and Information Sciences, Yokohama National University, Yokohama, 240-8501, Japan

    Hamako Sasamoto

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  1. Ai Hasegawa
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  2. Tomoya Oyanagi
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  3. Reiko Minagawa
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  4. Yoshiharu Fujii
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  5. Hamako Sasamoto
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Correspondence to Hamako Sasamoto.

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Hasegawa, A., Oyanagi, T., Minagawa, R. et al. An inverse relationship between allelopathic activity and salt tolerance in suspension cultures of three mangrove species, Sonneratia alba, S. caseolaris and S. ovata: development of a bioassay method for allelopathy, the protoplast co-culture method. J Plant Res 127, 755–761 (2014). https://doi.org/10.1007/s10265-014-0651-1

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  • DOI: https://doi.org/10.1007/s10265-014-0651-1

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