Regular paper

Journal of Plant Research

, Volume 126, Issue 3, pp 439-446

Transduction of pressure signal to electrical signal upon sudden increase in turgor pressure in Chara corallina

  • Teruo ShimmenAffiliated withDepartment of Life Science, Graduate School of Life Science, University of Hyogo Email author 
  • , Koreaki OgataAffiliated withSchool of Medicine, University of Occupational and Environmental Health

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Abstract

By taking advantage of large cell size of Chara corallina, we analyzed the membrane depolarization induced by decreased turgor pressure (Shimmen in J Plant Res 124:639–644, 2011). In the present study, the response to increased turgor pressure was analyzed. When internodes were incubated in media containing 200 mM dimethyl sulfoxide, their intracellular osmolality gradually increased and reached a steady level after about 3 h. Upon removal of dimethyl sulfoxide, turgor pressure quickly increased. In response to the increase in turgor pressure, the internodes generated a transient membrane depolarization at its nodal end. The refractory period was very long and it took about 2 h for full recovery after the depolarizing response. Involvement of protein synthesis in recovery from refractoriness was suggested, based on experiments using inhibitors.

Keywords

Chara Depolarization Dimethyl sulfoxide Ethylene glycol Membrane potential Osmolality Turgor pressure