Journal of Plant Research

, Volume 126, Issue 3, pp 439–446

Transduction of pressure signal to electrical signal upon sudden increase in turgor pressure in Chara corallina

Regular paper

DOI: 10.1007/s10265-012-0537-z

Cite this article as:
Shimmen, T. & Ogata, K. J Plant Res (2013) 126: 439. doi:10.1007/s10265-012-0537-z
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Abstract

By taking advantage of large cell size of Chara corallina, we analyzed the membrane depolarization induced by decreased turgor pressure (Shimmen in J Plant Res 124:639–644, 2011). In the present study, the response to increased turgor pressure was analyzed. When internodes were incubated in media containing 200 mM dimethyl sulfoxide, their intracellular osmolality gradually increased and reached a steady level after about 3 h. Upon removal of dimethyl sulfoxide, turgor pressure quickly increased. In response to the increase in turgor pressure, the internodes generated a transient membrane depolarization at its nodal end. The refractory period was very long and it took about 2 h for full recovery after the depolarizing response. Involvement of protein synthesis in recovery from refractoriness was suggested, based on experiments using inhibitors.

Keywords

CharaDepolarizationDimethyl sulfoxideEthylene glycolMembrane potentialOsmolalityTurgor pressure

Abbreviations

APW

Artificial pond water

APW(200 DMSO)

APW supplemented with 200 mM DMSO

CHI

Cycloheximide

DMSO

Dimethyl sulfoxide

EAB

Potential difference between pools A and B

EG

Ethylene glycol

Пi

Intracellular osmolality

Πo

Extracellular osmolality

Copyright information

© The Botanical Society of Japan and Springer Japan 2012

Authors and Affiliations

  1. 1.Department of Life Science, Graduate School of Life ScienceUniversity of HyogoHyogoJapan
  2. 2.School of MedicineUniversity of Occupational and Environmental HealthFukuokaJapan