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Identification of early induced genes upon water deficit in potato cell cultures by cDNA-AFLP

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Abstract

For plant cells in the early phases of water stress exposure, the genes induced under such conditions play a key role in detecting and responding to water deficit. In this study, potato cell suspensions were used as a simplified model system to dissect early molecular changes upon low water potential. In particular, the cDNA-amplified fragment length polymorphism approach was used to capture genes rapidly activated in potato cell cultures in response to water deficit induced by short-term exposure (up to 1 h) to polyethylene glycol. Selective amplifications with 38 primer combinations allowed the visualization of about 167 transcript-derived fragments (TDFs) differentially expressed upon exposure to low water potential. The gene expression pattern of 18 up-regulated genes was further investigated by semi-quantitative reverse transcriptase polymerase chain reaction analysis. Sequencing and similarity analysis revealed that TDFs present homologies chiefly with proteins involved in chaperone activity and protein degradation (hsps, proteinase precursor), in protein synthesis (elongation factor, ribosomal proteins) and in the ROS scavenging pathway (phenylalanine ammonia-lyase, peroxidase). Our findings might contribute to describe the potential role of genes activated in the early phases of plant response to drought.

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Acknowledgments

We express our thanks to Rosario Nocerino, Gaetano Guarino and Roberta Nurcato for excellent technical assistance and to Mark Walters for improving the quality of the manuscript. Contribution no. 371 of CNR-Institute of Plant Genetics (IGV), Italy.

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  1. Alfredo Ambrosone

    Present address: National Research Council of Italy, Institute of Cybernetics “E. Caianiello” (CNR-ICIB), Pozzuoli, Naples, Italy

  2. Michele Di Giacomo

    Present address: Research and Quality, Manifatture Sigaro Toscano, Lucca, Italy

Authors and Affiliations

  1. National Research Council of Italy, Institute of Plant Genetics (CNR-IGV), Via Università 133, Portici, Naples, Italy

    Alfredo Ambrosone, Michele Di Giacomo, M. Stefania Grillo & Antonello Costa

  2. Department of Pharmaceutical Sciences, University of Salerno, Fisciano, Salerno, Italy

    Antonella Leone

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  1. Alfredo Ambrosone
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Correspondence to Antonello Costa.

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Ambrosone, A., Di Giacomo, M., Leone, A. et al. Identification of early induced genes upon water deficit in potato cell cultures by cDNA-AFLP. J Plant Res 126, 169–178 (2013). https://doi.org/10.1007/s10265-012-0505-7

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