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Low nitrogen-induced expression of cyclophilin in Nicotiana tabacum

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Abstract

Leaf morphology and the leaf protein expression profiles of flue-cured tobacco grown in central Henan province of China under low nitrogen (low-N) and normal nitrogen (normal-N) nutrition were examined. The leaf length and width were measured at 50, 60, and 70 days after transplanting. Leaves grown under low-N conditions were shorter and more narrow than those grown under normal-N conditions. The protein expression profiles of tobacco leaves harvested at 70 days after transplanting were analyzed by 2-dimensional electrophoresis, and five differentially expressed proteins including a putative protein were identified. Except for the MCM protein-like protein, the other three differentially expressed proteins of cyclophilin-like protein, vacuolar invertase INV2, MAR-binding protein and the one putative protein showed increased expression in the low-N nutrition group. Among these proteins, the cyclophilin-like protein, which is a stress-responsive signal protein, may play pivotal roles in regulating leaf development under stress conditions. Real-time quantitative PCR analysis showed that the mRNA expression level of the cyclophilin-like protein at day 50, 60 and 70 under low-N conditions was 0.90, 1.43 and 6.9-fold higher than that under normal-N conditions, indicating that the gene expression of cyclophilin-like protein was strongly induced by low-N conditions.

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Acknowledgments

This work was supported by the grants of Major Project from State Tobacco Monopoly Administration of China (No. 110201101001) and Natural Science Fundamental Research Program of Henan Education Department (No. 2011A210019). We thank Dr. Xiaowen Yang in University of Leicester, UK and Dr. Neil Fannin in University of Kentucky, USA very much for the English writing revision.

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Authors and Affiliations

  1. College of Tobacco Science, Key Laboratory for Cultivation of Tobacco Industry, Henan Agricultural University, Zhengzhou, 450002, People’s Republic of China

    Huijuan Yang, Li Xu, Hong Cui, Guoshun Liu & Hongzhi Shi

  2. College of Animal Sciences, Zhejiang University, Hangzhou, 310029, People’s Republic of China

    Boxiong Zhong

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  1. Huijuan Yang
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  2. Li Xu
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  3. Hong Cui
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  4. Boxiong Zhong
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  5. Guoshun Liu
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  6. Hongzhi Shi
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Correspondence to Guoshun Liu or Hongzhi Shi.

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Yang, H., Xu, L., Cui, H. et al. Low nitrogen-induced expression of cyclophilin in Nicotiana tabacum . J Plant Res 126, 121–129 (2013). https://doi.org/10.1007/s10265-012-0499-1

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