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Effect of silicon deficiency on secondary cell wall synthesis in rice leaf

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Abstract

Rice (Oryza sativa L.) is a typical Si-accumulating plant and is able to accumulate Si up to >10 % of shoot dry weight. The cell wall has been reported to become thicker under Si-deficient condition. To clarify the relationship between Si accumulation and cell wall components, the physical properties of, and macromolecular components and Si content in, the pectic, hemicellulosic, and cellulosic fractions prepared from rice seedlings grown in hydroponics with or without 1.5 mM silicic acid were analyzed. In the absence of Si (the −Si condition), leaf blades drooped, but physical properties were enhanced. Sugar content in the cellulosic fraction and lignin content in the total cell wall increased under −Si condition. After histochemical staining, there was an increase in cellulose deposition in short cells and the cell layer just beneath the epidermis in the −Si condition, but no significant change in the pattern of lignin deposition. Expression of the genes involved in secondary cell wall synthesis, OsCesA4, OsCesA7, OsPAL, OsCCR1 and OsCAD6 was up-regulated under −Si condition, but expression of OsCesA1, involved in primary cell wall synthesis, did not increase. These results suggest that an increase in secondary cell wall components occurs in rice leaves to compensate for Si deficiency.

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Acknowledgments

This work was supported by a grant from Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics for Agricultural Innovation, GMA0007).

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8571, Japan

    Tsuyoshi Yamamoto, Atsuko Nakamura, Hiroaki Iwai, Tadashi Ishii, Shinobu Satoh & Jun Furukawa

  2. Forestry and Forest Products Research Institute, Matsunosato 1, Tsukuba, Ibaraki, 305-8687, Japan

    Tadashi Ishii

  3. Okayama University, Chuo 2-20-1, Kurashiki, Okayama, 710-0046, Japan

    Jian Feng Ma

  4. Tohoku University, Aoba 6-3, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8578, Japan

    Ryusuke Yokoyama & Kazuhiko Nishitani

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  1. Tsuyoshi Yamamoto
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  2. Atsuko Nakamura
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  3. Hiroaki Iwai
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  4. Tadashi Ishii
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  8. Shinobu Satoh
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  9. Jun Furukawa
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Correspondence to Jun Furukawa.

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Yamamoto, T., Nakamura, A., Iwai, H. et al. Effect of silicon deficiency on secondary cell wall synthesis in rice leaf. J Plant Res 125, 771–779 (2012). https://doi.org/10.1007/s10265-012-0489-3

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  • DOI: https://doi.org/10.1007/s10265-012-0489-3

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