Skip to main content
SpringerLink
Log in

Identification of parental genomes and genomic organization in Aster microcephalus var. ovatus

  • Regular Paper
  • Published:
Journal of Plant Research Aims and scope Submit manuscript

Abstract

The karyotype of diploid Aster iinumae is morphologically similar to that of diploid Aster ageratoides var. ageratoides, however, its chromosome size is apparently smaller (S-type chromosomes versus L-type chromosomes, respectively). The hybrid origin of tetraploid Aster microcephalus var. ovatus (LS-type chromosomes) has previously been suggested by cytogenetics and chloroplast DNA (cp DNA) data. The cp DNA phylogeny also implies that the S-type chromosome is apomorphic, which means that genome size reduction occurred on the evolutionary way to A. iinumae. In this study, we have demonstrated that the chromosome size difference does not depend on the intensity of chromosome condensation but on the DNA content. The simultaneous genomic in situ hybridization (GISH) results show the similarity between S-type chromosomes of A. iinumae and A. microcephalus var. ovatus, and between L-type chromosomes of A. ageratoides and A. microcephalus var. ovatus, which provide additional evidence for A. microcephalus var. ovatus being a tetraploid amphidiploid produced by hybridization between S-type chromosomes and L-type chromosomes. The distribution patterns of Ty1-copia-like retrotransposons were similar in L- and S-type chromosomes. The copies of this retrotransposon dispersed uniformly on all chromosomes, and it is not yet apparent how the Ty1-copia-like retrotransposon affects the size difference between them.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Ali HBM, Lysak MA, Schubert I (2004) Genomic in situ hybridization in plants with small genomes is feasible and elucidates the chromosomal parentage in interspecific Arabidopsis hybrids. Genome 40:954–960

    Article  Google Scholar 

  • Arnold ML (1997) Natural hybridization and evolution. Oxford University, Oxford

    Google Scholar 

  • Bennetzen JL (2000) Transposable element contributions to plant gene and genome evolution. Plant Mol Biol 42:251–269

    Article  PubMed  CAS  Google Scholar 

  • Bremer K (1994) Asteraceae. Cladistics & classification. Timber, Portland

    Google Scholar 

  • Brysting AK, Holst-Jensen A, Leitch I (2000) Genomic organization of the hybrid Poa jemtlandica (Poaceae) verified by genomic in situ hybridization and chloroplast DNA sequences. Ann Bot 85:439–445

    Article  CAS  Google Scholar 

  • Desel C, Jansen R, Dedong G, Schmidt T (2002) Painting of parental chromatin in beta hybrids by multi-color fluorescent in situ hybridization. Ann Bot 89:171–181

    Article  PubMed  Google Scholar 

  • Doležel J, Bartoš J (2005) Plant DNA flow cytometry and estimation of nuclear genome size. Ann Bot 95:99–110

    Article  PubMed  Google Scholar 

  • Flavell RB (1986) Repetitive DNA and chromosome evolution in plants. Phil Trans R Soc Lond B Biol Sci 312:227–242

    Article  CAS  Google Scholar 

  • Flavell AJ, Pearce SR, Heslop-Harrison JS, Kumar A (1997) The evolution of Ty1-copia group retrotransposons in eukaryote genomes. Genetica 100:185–195

    Article  PubMed  CAS  Google Scholar 

  • Galasso I, Harrison GE, Pignone D, Brandes A, Heslop-Harrison JS (1997) The distribution and organization of Ty1-copia-like retrotransposable elements in the genome of Vigna unguiculata (L.) Walp. (Cowpea) and its relatives. Ann Bot 80:327–333

    Article  CAS  Google Scholar 

  • Gatt M, Hammett K, Murray B (1999) Confirmation of ancient polyploidy in Dahlia (Asteraceae) species using genomic in situ hybridization. Ann Bot 84:39–48

    Article  Google Scholar 

  • Gu HY (1989) On chromosome numbers of Kalimeris (Astereae, Asteraceae) and some related taxa. Cathaya 1:1–16

    Google Scholar 

  • Hanson L, Brown RL, Boyd A, Johnson MAT, Bennett MD (2003) First nuclear DNA c-values for 28 angiosperm genera. Ann Bot 91:31–38

    Article  PubMed  CAS  Google Scholar 

  • Heslop-Harrison JS, Brandes A, Taketa S, Schmidt T, Vershinin AV, Alkhimova EG, Kamm A, Doudrick RL, Schwarzacher T, Katsiotis A, Kubis S, Kumar A, Pearce SR, Flavell AJ, Harrison GE (1997) The chromosomal distribution of Ty1-copia group retrotransposable elements in higher plants and their implications for genome evolution. Genetica 100:197–204

    Article  PubMed  CAS  Google Scholar 

  • Huziwara Y (1957a) Karyotype analysis in some genera of Compositae. II. The karyotype of Japanese Aster species. Cytologia 22:96–112

    Google Scholar 

  • Huziwara Y (1957b) Karyotype analysis in some genera of Compositae. III. The karyotype of the Aster ageratoides group. Am J Bot 44:783–790

    Article  Google Scholar 

  • Huziwara Y (1958) Karyotype analysis in some genera of Compositae. IV. The karyotypes within the genera Gymnaster, Kalimeris and Heteropappus. Cytologia 23:33–45

    Google Scholar 

  • Huziwara Y (1967) Chromosomal evolution in Aster and related genera. Taxon 16:303–304

    Article  Google Scholar 

  • Ito M, Soejima A, Hasebe M, Watanabe K (1995) A chloroplast-DNA phylogeny of Kalimeris and Aster, with reference to generic circumscription. J Plant Res 108:93–96

    Article  CAS  Google Scholar 

  • Ito M, Soejima A, Watanabe K (1998) Phylogenetic relationships of Japanese Aster (Asteraceae, Astereae) sensu lato based on chloroplast–DNA restriction site mutations. J Plant Res 111:217–223

    Article  CAS  Google Scholar 

  • Johnston JS, Pepper AE, Hall AE, Chen ZJ, Hodnett G, Drabek J, Lopez R, Price HJ (2005) Evolution of genome size in Brassicaceae. Ann Bot 95:229–235

    Article  PubMed  CAS  Google Scholar 

  • Kamm A, Doudrick RL, Heslop-Harrison JS, Schmidt T (1996) The genomic and physical organization of Ty1-copia-like sequences as a component of large genomes in Pinus elliottii var. elliottii and other gymnosperms. Proc Natl Acad Sci USA 93:2708–2713

    Article  PubMed  CAS  Google Scholar 

  • Kitamura S (1937) Compositae Japonicae. I. Mem Coll Sci Kyoto Imp Univ Ser B 13:337–357

    Google Scholar 

  • Leitch IJ, Heslop-Harrison JS (1992) Physical mapping of the 18S–5.8S–26S rRNA genes in barley by in situ hybridization. Genome 35:1013–1018

    CAS  Google Scholar 

  • Leitch IJ, Schwarzacher T, Jackson D, Leitch IJ (1994) In situ hybridization: a practical guide. BIOs Scientific, UK, pp 50–108

    Google Scholar 

  • Li CB, Zhang DM, Ge S, Lu BR, Hong DY (2001) Differentiation and inter genomic relationships among C, E and D genomes in the Oryza officinalis complex (Poaceae) as revealed by multicolor genomic in situ hybridization. Theor Appl Genet 103:197–203

    Article  CAS  Google Scholar 

  • Maluszynska J, Hasterok R (2005) Identification of individual chromosomes and parental genomes in Brassica juncea using GISH and FISH. Cytogenet Genome Res 109:310–314

    Article  PubMed  CAS  Google Scholar 

  • Marasek A, Hasterok R, Wiejacha K, Orlikowska T (2004) Determination by GISH and FISH of hybrid status in Lilium. Hereditas 140:1–7

    Article  PubMed  Google Scholar 

  • Matoba H, Soejima A, Hoshi Y, Kondo K (2005) Molecular cytogenetic organization of 5S and 18S rDNA loci in Aster ageratoides var. ageratoides, A. iinumae (=Kalimeris pinnatifida) and A. microcephalus var. ovatus in Japan. Cytologia 70:323–330

    Article  Google Scholar 

  • Meinkoth J, Wahl G (1984) Hybridization of nuclei acids immobilized on solid supports. Anal Biochem 138:267–284

    Article  PubMed  CAS  Google Scholar 

  • Miller JT, Dong F, Jackson SA, Song J, Jiang J (1998) Retrotransposon related DNA sequences in the centromeres of grass chromosomes. Genetics 150:1615–1623

    PubMed  CAS  Google Scholar 

  • Norrmann N, Hanson L, Renvoize S, Leitch IJ (2004) Genomic relationships among diploid and hexaploid species of Andropogon (Poaceae). Genome 47:1220–1224

    Article  PubMed  CAS  Google Scholar 

  • Pearce SR, Harrison G, Li D, Heslop-Harrison JS, Kumar A, Flavell AJ (1996) The Ty1-copia group retrotransposons in Vicia species: copy number, sequence heterogeneity and chromosomal location. Mol Gen Genet 250:305–315

    PubMed  CAS  Google Scholar 

  • Price HJ, Dillon SL, Hodnett G, Rooney W, Ross L, Johnston JS (2005) Genome evolution in the genus Sorghum (Poaceae). Ann Bot 95:219–227

    Article  PubMed  CAS  Google Scholar 

  • Refoufi A, Jahier J, Esnault MA (2001) Genomic analysis of Elytrigia pycnantha and Thinopyrum junceiforme and of their putative natural hybrid using the GISH technique. Genome 44:708–715

    Article  PubMed  CAS  Google Scholar 

  • Richard D, Rieseberg LH (1999) ITS sequence data support a single origin for North American Astereae (Asteraceae) and reflect deep geographic divisions in Aster s. l. Am J Bot 83:398–412

    Google Scholar 

  • Rieseberg LH (1997) Hybrid origins of plant species. Annu Rev Ecol Syst 28:359–389

    Article  Google Scholar 

  • Shaw CH (ed.) (1988) Plant molecular biology. A practical approach. Oxford University Press, Oxford

  • Shindo K (1967) Cytological, morphological and geographical studies on the differentiation of species in section Asteromoea of Kalimeris in Japan. J Sci Hiroshima Univ, Ser B Div 2 11:127–199

    Google Scholar 

  • Soltis PS, Soltis DE (1995) The role of genetic and genomic attributes in the success of polyploids. Proc Natl Acad Sci U S A 97:7051–7057

    Article  Google Scholar 

  • Soltis PS, Doyle JJ, Soltis DE (1992) Molecular data and polyploid evolution in plants. In: Soltis PS, Soltis DE, Doyle JJ (eds) Molecular systematics of plants, 177–201. Chapman and Hall, New York, p. 434

    Google Scholar 

  • Tara M (1977) Cytologenetic studies on natural intergeneric hybridization on Aster alliances. IV. Experimental confirmation of the hybrid origin of Aster ageratoides subsp. ovatus. Bot Mag Tokyo 90:253–258

    Article  Google Scholar 

  • Testolin R, Cipriani G (1997) Paternal inheritance of chloroplast DNA and maternal inheritance of mitochondrial DNA in the genus Actinidia. Theor Appl Genet 94:897–903

    Article  CAS  Google Scholar 

  • Vicient CM, Suoniemi A, Anamthawat-Jónsson K, Tanskanen J, Beharav A, Nevo E, Schulman AH (1999) Retrotransposon BARE-1 and its role in genome evolution in the genus Hordeum. Plant Cell 11:1769–1784

    Article  PubMed  CAS  Google Scholar 

  • Wendel JF, Cronn RC, Johnston JS, Price HJ (2002) Feast and famine in plant genomes. Genetica 115:37–47

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was financially supported by the Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Scientific Research (C), 2006, 18570094.

Author information

Authors and Affiliations

  1. Department of Applied Biological Science, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa, 252-8510, Japan

    Hideyuki Matoba

  2. School of Science, Osaka Prefecture University, Sakai, 599-8531, Japan

    Akiko Soejima

  3. Department of Plant Science, Kyushu Tokai University, Kawayo, Minamiaso-mura, Kumamoto, 869-1404, Japan

    Yoshikazu Hoshi

Authors
  1. Hideyuki Matoba
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Akiko Soejima
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yoshikazu Hoshi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yoshikazu Hoshi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Matoba, H., Soejima, A. & Hoshi, Y. Identification of parental genomes and genomic organization in Aster microcephalus var. ovatus . J Plant Res 120, 585–593 (2007). https://doi.org/10.1007/s10265-007-0101-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10265-007-0101-4

Keywords

Search

Navigation