Original Article

Journal of Plant Research

, Volume 118, Issue 1, pp 37-41

Nuclear DNA content in the genus Hepatica (Ranunculaceae)

  • Tomoo MabuchiAffiliated with2-11-5 Sagami-ohno
  • , Hisashi KokubunAffiliated withGraduate School of Science and Technology, Chiba University
  • , Masahiro MiiAffiliated withFaculty of Horticulture, Chiba University
  • , Toshio AndoAffiliated withFaculty of Horticulture, Chiba University Email author 

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Abstract

Using flow cytometry, we measured the nuclear DNA contents of all known taxa in Hepatica. Nuclear DNA content of Hepatica falconeri (diploid, crenate leaf lobes) was significantly lower than that of diploid entire species. Among the tetraploid species, crenate species had lower DNA contents than the entire taxon H. nobilis var. pubescens. The DNA content of the tetraploid species was more than double that of the diploid species among the same leaf-type groups.

Keywords

Anemone Flow cytometry Hepatica PI/DAPI ratio Ranunculaceae Speciation