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Beneficial effect of myricetin on renal functions in streptozotocin-induced diabetes

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Abstract

Myricetin is a naturally occurring flavonoid that is known to decrease plasma glucose levels in diabetes; however, its influence on renal functions has not yet been determined. This study investigated the effect of myricetin on structural and functional changes occurring in diabetic nephropathy. Male Albino Wistar rats were divided into three groups: normoglycemic, diabetic and myricetin-treated diabetic. Diabetes was induced by intraperitoneal (ip) injection of streptozotocin (50 mg/kg), and rats having fasting blood glucose (FBG) levels greater than 200 mg/dl were included in the study. Treatment of myricetin (6 mg/day ip) was initiated 16 weeks after diabetes was confirmed. Light microscopy was performed on hematoxylin–eosin- and Masson’s trichrome-stained sections to evaluate the effect of myricetin on structural changes in the kidney, while creatinine clearance, blood urea nitrogen (BUN), kidney weight, urine volume and protein were measured to assess kidney functions. Activities of glutathione peroxidase (GPx) and xanthine oxidase (XO) were also measured in renal tissues obtained from all experimental groups. Myricetin treatment significantly decreased glomerulosclerosis and reduced BUN, urinary volume and protein excretion, which was profoundly increased in diabetic rats. Decreased creatinine clearance measured in diabetic rats was significantly increased following myricetin treatment. Myricetin also restored altered renal activities of GPx and XO, which were decreased and increased in diabetic rats, respectively. In conclusion, myricetin improved altered renal functions and restored renal activities of GPx and XO in diabetic rats. Obtained data suggest that myricetin could be of therapeutic potential in diabetic nephropathy.

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References

  1. Alberti KG, Zimmet PZ (1998) Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med 15:539–553

    Article  PubMed  CAS  Google Scholar 

  2. Kanwar YS, Wada J, Sun L, Xie P, Wallner EI, Chen S, Chugh S, Danesh FR (2008) Diabetic nephropathy: mechanisms of renal disease progression. Exp Biol Med (Maywood) 233:4–11

    Article  CAS  Google Scholar 

  3. Gross JL, de Azevedo MJ, Silveiro SP, Canani LH, Caramori ML, Zelmanovitz T (2005) Diabetic nephropathy: diagnosis, prevention, and treatment. Diabetes Care 28:164–176

    Article  PubMed  Google Scholar 

  4. Xu Y, Osborne BW, Stanton RC (2005) Diabetes causes inhibition of glucose-6-phosphate dehydrogenase via activation of PKA, which contributes to oxidative stress in rat kidney cortex. Am J Physiol Renal Physiol 289:F1040–F1047

    Article  PubMed  CAS  Google Scholar 

  5. Kowluru RA, Abbas SN, Odenbach S (2004) Reversal of hyperglycemia and diabetic nephropathy: effect of reinstitution of good metabolic control on oxidative stress in the kidney of diabetic rats. J Diabetes Complications 18:282–288

    Article  PubMed  Google Scholar 

  6. Kamalakkannan N, Prince PS (2006) Antihyperglycaemic and antioxidant effect of rutin, a polyphenolic flavonoid, in streptozotocin-induced diabetic wistar rats. Basic Clin Pharmacol Toxicol 98:97–103

    Article  PubMed  CAS  Google Scholar 

  7. Ong KC, Khoo HE (1997) Biological effects of myricetin. Gen Pharmacol 29:121–126

    Article  PubMed  CAS  Google Scholar 

  8. Montoro P, Tuberoso CI, Piacente S, Perrone A, De Feo V, Cabras P, Pizza C (2006) Stability and antioxidant activity of polyphenols in extracts of Myrtus communis L. berries used for the preparation of myrtle liqueur. J Pharm Biomed Anal 41:1614–1619

    Article  PubMed  CAS  Google Scholar 

  9. Ong KC, Khoo HE (2000) Effects of myricetin on glycemia and glycogen metabolism in diabetic rats. Life Sci 67:1695–1705

    Article  PubMed  CAS  Google Scholar 

  10. Fabiny DL, Ertingshausen G (1971) Automated reaction-rate method for determination of serum creatinine with the CentrifiChem. Clin Chem 17:696–700

    PubMed  CAS  Google Scholar 

  11. Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169

    PubMed  CAS  Google Scholar 

  12. Beckman JS, Parks DA, Pearson JD, Marshall PA, Freeman BA (1989) A sensitive fluorometric assay for measuring xanthine dehydrogenase and oxidase in tissues. Free Radic Biol Med 6:607–615

    Article  PubMed  CAS  Google Scholar 

  13. Aliciguzel Y, Ozen I, Aslan M, Karayalcin U (2003) Activities of xanthine oxidoreductase and antioxidant enzymes in different tissues of diabetic rats. J Lab Clin Med 142:172–177

    Article  PubMed  CAS  Google Scholar 

  14. Wasowicz W, Nève J, Peretz A (1993) Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage. Clin Chem 39:2522–2526

    PubMed  CAS  Google Scholar 

  15. Raij L, Azar S, Keane WF (1985) Role of hypertension in progressive glomerular immune injury. Hypertension 7:398–404

    PubMed  CAS  Google Scholar 

  16. Akman S, Kalay S, Akkaya B, Koyun M, Akbaş H, Baysal YE, Guven AG (2009) Beneficial effect of triple treatment plus immunoglobulin in experimental nephrotic syndrome. Pediatr Nephrol 24:1173–1180

    Article  PubMed  Google Scholar 

  17. The Diabetes Control Complications Trial/Epidemiology of Diabetes Interventions, Complications Research Group (2000) Retinopathy and nephropathy in patients with type 1 diabetes four years after a trial of intensive therapy. N Engl JMed 342:381–389

    Google Scholar 

  18. Kang JJ, Toma I, Sipos A, McCulloch F, Peti-Peterdi J (2006) Quantitative imaging of basic functions in renal (patho) physiology. Am J Physiol Renal Physiol 291:F495–F502

    Article  PubMed  CAS  Google Scholar 

  19. Szkudelski T (2001) The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res 50:537–546

    PubMed  CAS  Google Scholar 

  20. Andreassen TT, Seyer-Hansen K, Oxlund H (1981) Biomechanical changes in connective tissues induced by experimental diabetes. Acta Endocrinol (Copenh) 98:432–436

    CAS  Google Scholar 

  21. Ong KC, Khoo HE (1996) Insulinomimetic effects of myricetin on lipogenesis and glucose transport in rat adipocytes but not glucose transport translocation. Biochem Pharmacol 51:423–429

    Article  PubMed  CAS  Google Scholar 

  22. Choi JS, Yokozawa T, Oura H (1991) Improvement of hyperglycemia and hyperlipemia in streptozotocin-diabetic rats by a methanolic extract of Prunus davidiana stems and its main component, prunin. Planta Med 57:208–211

    Article  PubMed  CAS  Google Scholar 

  23. Blondel O, Bailbe D, Portha B (1990) Insulin resistance in rats with non-insulin-dependent diabetes induced by neonatal (5 days) streptozotocin: evidence for reversal following phlorizin treatment. Metabolism 39:787–793

    Article  PubMed  CAS  Google Scholar 

  24. Shisheva A, Shechter Y (1992) Quercetin selectively inhibits insulin receptor function in vitro and the bioresponses of insulin and insulinomimetic agents in rat adipocytes. Biochemistry 31:8059–8063

    Article  PubMed  CAS  Google Scholar 

  25. Serradas P, Bailbé D, Blondel O, Portha B (1991) Abnormal B-cell function in rats with non-insulin-dependent diabetes induced by neonatal streptozotocin: effect of in vivo insulin, phlorizin, or vanadate treatments. Pancreas 6:54–62

    Article  PubMed  CAS  Google Scholar 

  26. Lavoie L, Dimitrakoudis D, Marette A, Annabi B, Klip A, Vranic M, van de Werve G (1993) Opposite effects of hyperglycemia and insulin deficiency on liver glycogen synthase phosphatase activity in the diabetic rat. Diabetes 42:363–366

    Article  PubMed  CAS  Google Scholar 

  27. Haneda M, Koya D, Kikkawa R (2001) Cellular mechanisms in the development and progression of diabetic nephropathy: activation of the DAG-PKC-ERK pathway. Am J Kidney Dis 38:S178–S181

    Article  PubMed  CAS  Google Scholar 

  28. Obrosova IG, Drel VR, Pacher P, Ilnytska O, Wang ZQ, Stevens MJ, Yorek MA (2005) Oxidative-nitrosative stress and poly(ADP-ribose) polymerase (PARP) activation in experimental diabetic neuropathy: the relation is revisited. Diabetes 54:3435–3441

    Article  PubMed  CAS  Google Scholar 

  29. Dave GS, Kalia K (2007) Hyperglycemia induced oxidative stress in type-1 and type-2 diabetic patients with and without nephropathy. Cell Mol Biol (Noisy-le-grand) 53:68–78

    CAS  Google Scholar 

  30. Triggiani V, Resta F, Guastamacchia E, Sabbà C, Licchelli B, Ghiyasaldin S, Tafaro E (2006) Role of antioxidants, essential fatty acids, carnitine, vitamins, phytochemicals and trace elements in the treatment of diabetes mellitus and its chronic complications. Endocr Metab Immune Disord Drug Targets 6:77–93

    Article  PubMed  CAS  Google Scholar 

  31. Derosa G, Maffioli P (2010) Effects of thiazolidinediones and sulfonylureas in patients with diabetes. Diabetes Technol Ther 12:491–501

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by a grant (No: 2007.02.0122.004) from Akdeniz University Research Foundation.

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The authors declare that they have no financial and non-financial conflict of interest.

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Correspondence to Mutay Aslan.

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Ozcan, F., Ozmen, A., Akkaya, B. et al. Beneficial effect of myricetin on renal functions in streptozotocin-induced diabetes. Clin Exp Med 12, 265–272 (2012). https://doi.org/10.1007/s10238-011-0167-0

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  • DOI: https://doi.org/10.1007/s10238-011-0167-0

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