Abstract
Mining generates large amounts of wastewater, which may contain elevated levels of potentially toxic metals. We evaluated the use of live cells of Mesorhizobium amorphae CCNWGS0123 as a new, relatively inexpensive and environment-friendly adsorbent material for removal of toxic metals from aqueous solutions, such as mine tailings leachate. Maximum copper uptake and removal efficiency was attained at a dosage of 0.5 g L−1, a pH of 5.0, an agitation speed of 150 rpm, and at a temperature of 28 °C for an initial Cu2+ concentration of 100 mg L−1; equilibrium was achieved within 30 min. The adsorption isothermal data matched Langmuir and Freundlich isotherms well, with correlation coefficients of 0.931 and 0.932, respectively. FT-IR analysis indicated that many functional groups with a negative charge on the cell surface were involved in the interaction between live CCNWGS0123 and copper ions. Scanning electron microscope results confirmed that there was some copper sediment on the cell surface, causing deformation, aggregation, and cell surface damage. Cu2+ accumulation was related to cell growth, and copper uptake was not only due to cell-surface binding, but also to intracellular accumulation. Live cells of CCNWGS0123 are a new biosorbent that have the potential to be economical when small batches of biomass are used to remove Cu2+ from an aqueous solution.
Bergbau bringt große Mengen Abwässer hervor, das erhöhte Konzentrationen potenziell toxischer Metalle enthalten kann. Wir untersuchten die Verwendung lebender Zellen von Mesorhizobium amorphae CCNWGS0123 als ein neues, relativ kostengünstiges und umweltfreundliches Absorbent zur Entfernung toxischer Metalle aus wässrigen Lösungen, wie beispielsweise Sickerwasser aus Abraumhalden. Dabei wurde die maximale Kupferaufnahme und -entfernung bei einer Dosierung von 0.5 g/L, einem pH-Wert von 5,0, einer Rührgeschwindigkeit von 150 Umdrehungen pro Minute und einer Temperatur von 28 °C mit einer anfänglichen Cu (II) Konzentration von 100 mg/L erzielt. Das Gleichgewicht stellte sich nach 30 min ein. Wir konnten zeigen, dass die Adsorptionsisothermen sehr gut mit Langmuir und Freundlich-Isothermen korrelierten und entsprechende Korrelationskoeffizienten von 0,931 und 0,932 besitzen. Wie die FT-IR Analyse belegte sind viele funktionelle Gruppen mit negativer Ladung auf der Zelloberfläche in die Wechselwirkung von lebenden CCNWGS0123 mit den Kupferionen eingebunden. Weiterhin belegten die Ergebnisse der Rasterelektronenmikroskopie, dass die Kupferablagerungen auf der Zelloberfläche eine Deformierung, Zusammenballung sowie Schäden an der Zelloberfläche verursachten. Die Cu2+-Anreicherung war an das Zellwachstum gebunden und Kupfer wurde nicht nur durch Zelloberflächenbindung aufgenommen, sondern auch interzellulär angereichert. Lebende CCNWGS0123-Zellen sind daher ein neues Biosorptionsmittel, das potentiell wirtschaftlich eingesetzt werden kann, wenn kleine Mengen Biomasse verwandt werden um Cu2+ aus wässrigen Lösungen zu entfernen.
Resumen
La minería genera grandes cantidades de aguas residuales que contienen elevados niveles de metales potencialmente tóxicos. Hemos usado células viable de Mesorhizobium amorphae CCNWGS0123 como un material adsorbente nuevo, relativamente barato y ambientalmente amigable para la remoción de metales tóxicos desde soluciones acuosas, tales como los lixiviados de colas de mina. La máxima eficiencia en la remoción de cobre fue alcanzada a una concentración de 0.5 g L−1, un pH de 5,0, una velocidad de agitación de 150 rpm, y a una temperatura de 28 °C para una concentración inicial de Cu (II) de 100 mg L−1; el equilibrio fue alcanzado dentro de los 30 min. Los datos de las isotermas de adsorción ajustaron con las isotermas de Langmuir y Freundlich, con coeficientes de correlación de 0,931 y 0,932, respectivamente. El análisis por FT-IR indicó que muchos grupos funcionales con carga negativa sobre la superficie celular estuvieron involucrados en la interacción entre CCNWGS0123 y los iones cobre. Los resultados SEM mostraron que hubo algún sedimento de cobre sobre la superficie celular, causando deformación, agregado y daño de la superficie celular. La acumulación de Cu2+ estuvo vinculada al crecimiento celular y la retención de cobre fu debido no sólo a la unión con la superficie sino también a la acumulación intracelular. Las células viables de CCNWGS0123 son un nuevo biosorbente que el potencial de ser económico cuando es usado para remover Cu2+ desde una solución acuosa.
采矿会产生大量富含高浓度毒性金属离子的矿山废水。我们用活体Mesorhizobium amorphae CCNWGS0123细胞作为新型、低成本、环境友好的尾矿淋滤液有毒金属离子吸附剂。在剂量为0.5 g L−1、pH等于5、搅动速度150 rpm、温度28 °C、Cu(II)初始浓度100 mg L−1的条件,反应能够在30分钟内达到平衡,并可获得最大铜离子吸附和去除效率。该反应的吸附等温曲线与Langmuir和Freundlich等温曲线吻合,相关系数分别为0.931和0.932。傅立叶变换红外光谱(FT-IR)分析表明,细胞表面的多种功能团及负电荷参与了活体CCNWGS0123细胞与Cu离子之间的反应。扫描电子显微镜证明,铜在细胞表面的沉积引起了细胞的变形、聚合和表面破坏。C2 + 的富集与去除作用与细胞生长有关,是细胞表面束缚与细胞内积聚共同作用的结果。当需要小型生物群落用于去除溶液中Cu2 + 时,活体CCNWGS0123细胞可作为新型、经济的生物吸附剂。.
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Acknowledgments
This work was supported by projects from National Science Foundation of China (31125007, 30970003 and 31070444), and the 863 project of China (2012AA101402). The authors are grateful to Prof. Dr. Abdelshafi Abdelsamee from Suez Canal University; Egypt, Mr. Qiang Baifa from North West Agriculture and Forestry University and to the staff in international school of North West Agriculture and Forestry University in China for there supports. Also, special thanks go to the Department of International Cooperation of the ministry of Science and Technology (MOST) for participation in international training workshops in China.
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Mohamad, O.A., Hatab, S.R., Liu, Z. et al. Biosorption and Bioaccumulation of Cu2+ from Aqueous Solution Using Living M. amorphae Isolated from Mine Tailings. Mine Water Environ 31, 312–319 (2012). https://doi.org/10.1007/s10230-012-0187-3
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DOI: https://doi.org/10.1007/s10230-012-0187-3