Abstract
Genetic characterization was performed in five individuals of wild Amur sturgeon Acipenser schrenckii, and/or its presumed hybrid caught around Hokkaido, using a mitochondrial DNA (mtDNA) marker and two markers of nuclear DNA (nDNA). Genetic analyses indicated that two of the five fish had the mtDNA haplotype of Kaluga, Huso dauricus, whereas the nDNA markers indicated signs for both A. schrenckii and H. dauricus genotypes, referring to a hybrid origin. The other three fish were plausibly pure A. schrenckii. The results indicated the importance of combined usage of mtDNA and nDNA markers for correct species identification in sturgeon.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barmintseva AE, Mugue NS (2013) The use of microsatellite loci for identification of sturgeon species (Acipenseridae) and hybrid forms. Genetika 49:1093–1105
Billard R, Lecointre G (2001) Biology and conservation of sturgeon and paddlefish. Rev Fish Biol Fish 10:355–392
Birstein VJ (1993) Sturgeons and Paddlefishes: Threatened Fishes in Need of Conservation. Cons Biol 7:773–787
Burgener M, Hübner P (1998) Mitochondrial DNA enrichment for species identification and evolutionary analysis. Z Lebensm Unters Forsch A 207:261–263
Dudu A, Suciu R, Paraschiv M, Georgescu SE, Costache M, Berrebi P (2011) Nuclear markers of Danube sturgeons hybridization. Int J Mol Sci 12:6796–6809
Dupuis JR, Roe AD, Sperling FAH (2012) Multi-locus species delimitation in closely related animals and fungi: one marker is not enough. Mol Ecol 21:4422–4436
Field KG, Olsen GJ, Lane DJ, Giovannoni S, Ghiselin MT, Raff EC, Pace NR, Raff RA (1988) Molecular phylogeny of the animal kingdom. Science 239:748–753
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 41: 95–98
Havelka M, Kašpar V, Hulák M, Flajšhans M (2011) Sturgeon genetics and cytogenetics: a review related to ploidy levels and interspecific hybridization. Folia Zool 60:93–103
Havelka M, Hulák M, Ráb P, Rábová M, Lieckfeldt D, Ludwig A, Rodina M, Gela D, Pšenička M, Bytyutskyy D, Flajšhans M (2014) Fertility of a spontaneous triploid Siberian sturgeon, Acipenser baerii. BMC Genet 15:5
Honma Y (1988) Records and distributional notes on the sturgeons along the coast of Japanese archipelago. Bull Biogeographical Soc Japan 43:51–55
Jenneckens I (1999) Investigations on biochemical and molecular markers on their suitability in the identification of sturgeons and their hybrids. PhD dissertation, Universität Göttingen.
Kjer KM (2004) Aligned 18S and insect phylogeny. Syst Biol 53:506–514
Krieger J and Fuerst PA (2004) Diversity of nuclear 18S rRNA gene sequences within individuals in lake sturgeon (Acipenser fulvescens). J Appl Ichthyol 20:433–439
Krieger J, Hett AK, Fuerst PA, Birstein VJ, Ludwig A (2006) Unusual intraindividual variation of the nuclear 18S rRNA gene is widespread within the acipenseridae. J Hered 97:218–225
Li J, Liu D, Ma Q, Zhan X, Dai W, Chen Y, Liu Y, Song Z (2015) Discriminating Dabry’s sturgeon (Acipenser dabryanus) and Chinese sturgeon (A. sinensis) based on DNA barcode and six nuclear markers. Hydrobiologia 757:185–196
Ludwig A, Lippold S, Debus L, Reinartz R (2009) First evidence of hybridization between endangered sterlets (Acipenser ruthenus) and exotic Siberian sturgeons (Acipenser baerii) in the Danube River. Biol Invasions 11:753–760
Omoto N, Maebayashi M, Hara A, Adachi S, Yamauchi K (2004) Gonadal maturity in wild sturgeons, Huso dauricus, Acipenser mikadoi and A. schrenckii caught near Hokkaido, Japan. Environ Biol Fish 70:381–391
Pikitch EK, Doukakis P, Lauck L, Chakrabarty P, Erickson DL (2005) Status, trends and management of sturgeon and paddlefish fisheries. Fish Fish 6:233–265
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nature Biotech 18:233–234
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6:Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30:2725–2729
Wei QW, Zou Y, Li P, Li L. (2011) Sturgeon aquaculture in China: progress, strategies and prospects assessed on the basis of nation-wide surveys (2007–2009). J Appl Ichthyol 27:162–168
Xia X, Xie Z, Kjer KM (2003) 18S ribosomal RNA and tetrapod phylogeny. Syst Biol 52:283–295
Zane L, Patarnello T, Ludwig A, Fontana F, Congiu L (2002) Isolation and characterization of microsatellites in the Adriatic sturgeon (Acipenser naccarii). Mol Ecol Notes 2:586–588
Zhang X, Wu W, Li L, Ma X, Chen J (2013) Genetic variation and relationships of seven sturgeon species and ten interspecific hybrids. Genet Sel Evol 45:2–10
Acknowledgments
This study was partly supported by the Regional Innovation Strategy Support Program, Ministry of Education, Culture, Sports, Science, and Technology, Japan, and funded by JSPS KAKENHI Grant Number 26450256. The research presented here complies with the current laws of Japan and the guidelines of the Ichthyological Society of Japan. The authors declare no conflicts of interest.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Azuma, N., Hagihara, S., Ichimura, M. et al. Genetic characterization of Amur sturgeon Acipenser schrenckii and its hybrid caught around Hokkaido. Ichthyol Res 64, 139–144 (2017). https://doi.org/10.1007/s10228-016-0544-5
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10228-016-0544-5