Abstract
MicroRNAs (miRNAs) are known to influence ovarian cell proliferation, apoptosis and hormone release, but it remains unknown whether miRNAs affect ovarian functions via transcription factors. We examined the effect of miRNAs on nuclear factor-κappaB (NF-kB) (p65) expression in human ovarian luteinized granulosa cells. We transfected cultured primary human ovarian luteinized granulosa cells with 80 different constructs encoding human pre-miRNAs and then evaluated NF-kB (p65) expression (percentage of cells containing p65) by immunocytochemistry. We found that 21 of the constructs stimulated NF-kB (p65) expression and 18 of the constructs inhibited NF-kB (p65) expression. This is the first direct demonstration that miRNAs affect NF-kB (p65) expression and the first genome-scale miRNA screen to identify upregulation and downregulation of NF-kB accumulation by miRNAs in the ovary. Novel miRNAs that affect the NF-kB signalling pathway could be useful for the control of NF-kB-dependent reproductive processes and the treatment of NF-kB-dependent reproductive disorders.
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Acknowledgments
The authors express their deep gratitude to Mrs. K. Tothová and Ž. Kuklová for skilful technical assistance. The present studies were supported by the Ministry of Agriculture of the Slovak Republic (projects RVVU 07–02 and RVVU 07–013). A.H. Harrath, S.H. Alwasel and A.V. Sirotkin extend their appreciation to the Deanship of Scientific Research at King Saud University for funding this work through the research group project NoRGP-VPP-164.
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Sirotkin, A.V., Alexa, R., Kišová, G. et al. MicroRNAs control transcription factor NF-kB (p65) expression in human ovarian cells. Funct Integr Genomics 15, 271–275 (2015). https://doi.org/10.1007/s10142-014-0413-0
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DOI: https://doi.org/10.1007/s10142-014-0413-0