Functional & Integrative Genomics

, Volume 12, Issue 4, pp 635–647

Genome-wide identification of rice class I metallothionein gene: tissue expression patterns and induction in response to heavy metal stress

  • Neelam Gautam
  • Pankaj Kumar Verma
  • Shikha Verma
  • Rudra Deo Tripathi
  • Prabodh Kumar Trivedi
  • Bijan Adhikari
  • Debasis Chakrabarty
Original Paper

DOI: 10.1007/s10142-012-0297-9

Cite this article as:
Gautam, N., Verma, P.K., Verma, S. et al. Funct Integr Genomics (2012) 12: 635. doi:10.1007/s10142-012-0297-9

Abstract

Metallothioneins (MTs) are members of a family of cysteine-rich low molecular weight polypeptides which play an important role in heavy metal detoxification and homeostasis of intracellular metal ions in plant. Though MT genes from some selected plants have been characterized with respect to their protein sequences, kinetic properties and tissue-specific localization, no detailed study has been carried out in rice. Here, we present genome-wide identification, structural and expression analyses of rice MT gene family. Our analysis suggests presence of 11 class I MT genes in rice genome (Release 7 of the MSU Rice Genome Annotation Project) which are differentially expressed during growth and development, in various tissues and during biotic and abiotic stresses. Our analyses suggest that class I MT proteins in rice differ in tissue localization as well as in heavy metal coordination chemistry. We also suggest that some MTs have a predominant role in detoxification of As (V) in arsenic-tolerant rice cultivars. Our analysis suggests that apart from transcriptional regulation, post-transcriptional alternative splicing in some members of this family takes place during growth and development, in various tissues and during biotic and abiotic stresses.

Keywords

Alternative splicingArsenicHeavy metalRiceStressMetallothionein

Supplementary material

10142_2012_297_MOESM1_ESM.ppt (126 kb)
Supplementary Fig. S1Possibility for tandem duplication was analysed using Vista Tools for Comparative Genomics (Frazer et al. 2004). Our analysis suggests that Os12g38010 and Os12g38051 are tandem duplication in the rice genome. (PPT 126 kb)
10142_2012_297_MOESM2_ESM.doc (36 kb)
Supplementary Table S1OsMT class I gene family specific primers used in the present experiment for qRT-PCR designed by using Primique online software http://cgi-www.daimi.au.dk/cgi-chili/primique/front.py. (DOC 35 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Neelam Gautam
    • 1
  • Pankaj Kumar Verma
    • 1
  • Shikha Verma
    • 1
  • Rudra Deo Tripathi
    • 1
  • Prabodh Kumar Trivedi
    • 1
  • Bijan Adhikari
    • 2
  • Debasis Chakrabarty
    • 1
  1. 1.CSIR-National Botanical Research InstituteLucknowIndia
  2. 2.Rice Research Station, Department of AgricultureHooghlyIndia