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Wheat defense genes in fungal (Puccinia striiformis) infection

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Abstract

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases of wheat worldwide. To isolate defense-related genes against the pathogen, a suppression subtractive hybridization library was constructed for an incompatible interaction. From the library, 652 sequences were determined to be unigenes, of which 31 were determined as genes involved in signal transduction and 77 were predicted to encode defense-related proteins. Expression patterns of 12 selected signal transduction and defense-related genes were determined using quantitative real-time polymerase chain reaction. Signal transduction genes started increasing their expression at 12 h post inoculation (hpi), and expressions of the most of the transport and resistance-related genes were induced at 18 hpi. The gene expression results indicate specific molecular and cellular activities during the incompatible interaction between wheat and the stripe rust pathogen. In general, the expression increase of wheat signal transduction genes soon after inoculation with the pathogen inducing various defense-related genes, including reactive oxygen species, ATP-binding cassette (ABC) transporters, pathogenesis-related proteins, and genes involved in the phenylpropanoid pathway. The activities of these defense genes work in a sequential and concerted manner to result in a hypersensitive response.

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Acknowledgements

This study was supported financially by the National Basic Research Program of China (No. 2006CB101901), the earmarked fund for Modern Agro-Industry Technology Research System, the Program for Changjiang Scholars and Innovative Research Team in University, Ministry of Education of China (No. 200558), the Nature Science Foundation of China (No. 30930064), and the 111 Project from Ministry of Education of China (B07049).

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  1. Xiumei Yu

    Present address: College of Life Science, Agricultural University of Hebei, Baoding, Hebei, 071001, People’s Republic of China

Authors and Affiliations

  1. College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, People’s Republic of China

    Xiumei Yu, Xiaojie Wang, Chenfang Wang, Zhipeng Qu, Xiudao Yu, Qingmei Han, Jie Zhao, Jun Guo, Lili Huang & Zhensheng Kang

  2. USDA-ARS and Department of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA

    Xianming Chen

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Correspondence to Zhensheng Kang.

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Yu, X., Wang, X., Wang, C. et al. Wheat defense genes in fungal (Puccinia striiformis) infection. Funct Integr Genomics 10, 227–239 (2010). https://doi.org/10.1007/s10142-010-0161-8

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