Abstract:
We describe the isolation of microorganisms utilizing fructosyl-amine (Amadori compound) from the marine environment and of fructosyl-amine oxidase from a marine yeast. Using fructosyl-valine (Fru-Val), a model Amadori compound for glycated hemoglobin, we isolated 12 microbial strains that grow aerobically in a minimal medium supplemented with Fru-Val as the sole nitrogen source. Among these strains, a yeast strain identified as Pichia sp. N1-1, produced a Fru-Val–oxidizing enzyme. The enzyme was purified in its active form, a single-polypeptide water-soluble protein of 54 kDa by gel electrophoresis, producing H2O2 with the oxidation of Fru-Val. By its substrate specificity, the enzyme was categorized as a novel fructosyl-amine oxidase. This is the first study on the isolation of microorganisms utilizing fructosyl-amine in the marine environment and of fructosyl-amine oxidase from budding yeast.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received October 21, 1999; accepted September 12, 2000
Rights and permissions
About this article
Cite this article
Sode, K., Ishimura, F. & Tsugawa, W. Screening and Characterization of Fructosyl-Valine–Utilizing Marine Microorganisms. Mar. Biotechnol. 3, 126–132 (2001). https://doi.org/10.1007/s101260000065
Issue Date:
DOI: https://doi.org/10.1007/s101260000065