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RNA-Seq Analysis of the Antioxidant Status and Immune Response of Portunus trituberculatus Following Aerial Exposure

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Abstract

Desiccation tolerance has been long considered as an important trait for the life survival under acute environmental stress. One of the biggest problems for modern commercial crab farming is desiccation during transportation; high mortality could occur following the aerial exposure. In this regard, here, we utilized RNA-seq-based transcriptome profiling to characterize the molecular responses of swimming crab in response to aerial exposure. In present study, following aerial exposure, the gill samples were sequenced at 0, 6, 12, and 18 h. And the sequenced reads were assembled into 274,594 contigs, with average length of 735.59 bp and N50 size of 1262 bp. After differential expression analysis, a total of 1572 genes were captured significantly differentially expressed, and were categorized into antioxidant/oxidative stress response, chaperones/heat shock proteins, immune alteration, cell proliferation/apoptosis, and cytoskeletal. Our analysis revealed the dramatic tissue oxidant stress and the alteration of the tissue epithelial integrity, especially many genes that have not been reported in crab species. With the limited functional information in crab, further studies are needed and underway in our lab to further characterize the key cellular actors governing the crab tolerance to aerial exposure. Taken together, our results provide molecular resources for further identification of key genes for desiccation tolerance, and to facilitate the molecular selection and breeding of desiccation tolerant strain and family.

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Acknowledgements

This project was supported by the Shrimp & Crab Innovation Team of Shandong Agriculture Research System (SDAIT-15-011), the science and technology plan of Qingdao (No. 14-2-4-87-jch), the national natural science foundation of China (No. 31101916), and was also supported by the Advanced Talents Foundation of QAU grant (Grant No.: 663–1114337).

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Authors and Affiliations

  1. Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China

    Yuquan Li, Shoumin Lai, Renjie Wang, Yuchao Zhao, Hao Qin, Lingxu Jiang, Na Li & Chao Li

  2. State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai, 200062, China

    Qiang Fu

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  1. Yuquan Li
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  2. Shoumin Lai
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  7. Na Li
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  8. Qiang Fu
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  9. Chao Li
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Correspondence to Chao Li.

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Li, Y., Lai, S., Wang, R. et al. RNA-Seq Analysis of the Antioxidant Status and Immune Response of Portunus trituberculatus Following Aerial Exposure. Mar Biotechnol 19, 89–101 (2017). https://doi.org/10.1007/s10126-017-9731-2

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