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Biochemical Modulation by Carbon and Nitrogen Addition in Cultures of Dictyota menstrualis (Dictyotales, Phaeophyceae) to Generate Oil-based Bioproducts

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Abstract

Dictyota menstrualis (Hoyt) Schnetter, Hörning & Weber-Peukert (Dictyotales, Phaeophyceae) was studied for the production of oil-based bioproducts and co-products. Experiments were performed to evaluate the effect of carbon dioxide (CO2) concentration, under nitrogen (NO3 −) limiting and saturation conditions, on growth rate (GR), photosynthesis, as well as nitrate reductase (NR), carbonic anhydrase (CA), and Rubisco activities. In addition, the biochemical composition of D. menstrualis under these conditions was estimated. GR, protein content, and N content in D. menstrualis were higher in treatments containing NO3 −, irrespective of CO2 addition. However, when CO2 was added to medium saturated with NO3 −, values of maximum photosynthesis, Rubisco, and NR activity, as well as total soluble carbohydrates and lipids, were increased. CA activity did not vary under the different treatments. The fatty acid profile of D. menstrualis was characterized by a high content of polyunsaturated fatty acids, especially the omega-3 fatty acids, making it a possible candidate for nutraceutical use. In addition, this species presented high GR, photosynthetic rate, and fatty acid content, highlighting its economic importance and the possibility of different biotechnological applications.

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Acknowledgments

This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) [projects 10/50193-1 and 12/19844-1], Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) [by scholarships provided to the first author], Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil), and the Ministério da Saúde, Ministério de Ciência e Tecnologia and CNPq-INCT-Redoxoma.

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  1. Instituto de Química, Departamento de Bioquímica, Universidade de São Paulo, Caixa Postal 26077, 05599-970, São Paulo, SP, Brazil

    Aline P. Martins & Pio Colepicolo

  2. Núcleo de Pesquisa em Ficologia, Instituto de Botânica, Secretaria do Meio Ambiente do Estado de São Paulo, Av. Miguel Estéfano, 3687, 04301-012, São Paulo, SP, Brazil

    Nair S. Yokoya

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  1. Aline P. Martins
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Correspondence to Aline P. Martins.

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Martins, A.P., Yokoya, N.S. & Colepicolo, P. Biochemical Modulation by Carbon and Nitrogen Addition in Cultures of Dictyota menstrualis (Dictyotales, Phaeophyceae) to Generate Oil-based Bioproducts. Mar Biotechnol 18, 314–326 (2016). https://doi.org/10.1007/s10126-016-9693-9

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