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Mapping QTL for Resistance Against Viral Nervous Necrosis Disease in Asian Seabass

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Abstract

Viral nervous necrosis disease (VNN), caused by nervous necrosis virus (NNV), leads to mass mortality in mariculture. However, phenotypic selection for resistance against VNN is very difficult. To facilitate marker-assisted selection (MAS) for resistance against VNN and understanding of the genetic architecture underlying the resistance against this disease, we mapped quantitative trait loci (QTL) for resistance against VNN in Asian seabass. We challenged fingerlings at 37 days post-hatching (dph), from a single back-cross family, with NNV at a concentration of 9 × 106 TCID50/ml for 2 h. Daily mortalities were recorded and collected. A panel of 330 mortalities and 190 surviving fingerlings was genotyped using 149 microsatellites with 145 successfully mapped markers covering 24 linkage groups (LGs). Analysis of QTL for both resistance against VNN and survival time was conducted using interval mapping. Five significant QTL located in four LGs and eight suggestive QTL in seven LGs were identified for resistance. Another five significant QTL in three LGs and five suggestive QTL in three LGs were detected for survival time. One significant QTL, spanning 3 cM in LG20, was identified for both resistance and survival time. These QTL explained 2.2–4.1 % of the phenotypic variance for resistance and 2.2–3.3 % of the phenotypic variance for survival time, respectively. Our results suggest that VNN resistance in Asian seabass is controlled by many loci with small effects. Our data provide information for fine mapping of QTL and identification of candidate genes for a better understanding of the mechanism of disease resistance.

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Acknowledgements

We thank Professor Jimmy Kwang and the Agri-food and Veterinary Authority of Singapore (AVA) for providing the NNV and Asian seabass cell lines. The project was supported by the National Research Foundation, Prime Minister’s Office, Singapore under its Competitive Research Program (CPR Award No. NRF-CPR7-2010-01) and the Singapore Ministry of Education Tier 1 grant through R-154-000-593-112.

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Authors and Affiliations

  1. Department of Biological Sciences, National University of Singapore, 14 Science Drive, Singapore, 117543, Singapore

    Peng Liu, Sek-Man Wong & Gen Hua Yue

  2. Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore, 117604, Singapore

    Peng Liu, Le Wang, Zi Yi Wan, Bao Qing Ye, Shuqing Huang, Sek-Man Wong & Gen Hua Yue

  3. National University of Singapore Suzhou Research Institute, Suzhou, Jiangsu, 215123, China

    Sek-Man Wong

  4. School of Biological Sciences, Nanyang Technological University, 6 Nanyang Drive, Singapore, 637551, Singapore

    Gen Hua Yue

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  1. Peng Liu
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Table S1

149 microsatellite markers used for mapping QTL in Asian seabass. (DOCX 56 kb)

Table S2

The reconstructed linkage groups of Asian seabass. (DOCX 18 kb)

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Liu, P., Wang, L., Wan, Z.Y. et al. Mapping QTL for Resistance Against Viral Nervous Necrosis Disease in Asian Seabass. Mar Biotechnol 18, 107–116 (2016). https://doi.org/10.1007/s10126-015-9672-6

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