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RUNX3 expression is associated with sensitivity to pheophorbide a-based photodynamic therapy in keloids

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Abstract

Runt-related transcription factor 3 (RUNX3) has recently been reported to be a possible predictor of sensitivity of cancer cells for photodynamic therapy (PDT), a promising therapeutic modality for keloids. In this study, we aimed to elucidate the implications of RUNX3 for keloid pathogenesis and sensitivity to pheophorbide a-based PDT (Pa-PDT). RUNX3 and proliferating cell nuclear antigen (PCNA) expression were examined in 6 normal skin samples and 32 keloid tissue samples by immunohistochemistry. We found that RUNX3 expression was detected more often in keloid tissues than in dermis of normal skin. In keloid tissues, RUNX3 expression was significantly increased in patients presenting with symptoms of pain or pruritus, and was also significantly related to PCNA expression. The therapeutic effect of Pa-PDT was comparatively investigated in keloid fibroblasts (KFs) with and without RUNX3 expression. Significant differences were found after Pa-PDT between KFs with and without RUNX3 expression in cell viability, proliferative ability, type I collagen expression, generation of reactive oxygen species (ROS), and apoptotic cell death. In addition, RUNX3 expression was significantly decreased after Pa-PDT in KFs, and KFs with downregulation of RUNX3 showed significantly increased cell viability after Pa-PDT. Pa-PDT may be a potential therapeutic modality for keloids, and RUNX3, as a possible contributor to keloid pathogenesis, may improve sensitivity to Pa-PDT in KFs.

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Acknowledgments

The research was supported by the National Natural Science Foundation of China (NSFC, no. 81260233), and a faculty research grant from Yonsei University College of Medicine for 2012 (6-2012-0180).

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Authors and Affiliations

  1. Department of Dermatology, Yanbian University Hospital, Yanji City, Jilin Province, China

    Zhenlong Zheng, Lianhua Zhu, Lianhua Li & Zhehu Jin

  2. Department of Dermatology, Yonsei University College of Medicine, Seoul, South Korea

    Mi Ryung Roh

  3. Department of Pathology, Yanbian University Hospital, Yanji City, Jilin Province, China

    Xianglan Zhang

  4. Oral Cancer Research Institute, College of Dentistry, Yonsei University, Seoul, South Korea

    Xianglan Zhang & Sook Moon

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  1. Zhenlong Zheng
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  2. Lianhua Zhu
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  3. Xianglan Zhang
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  4. Lianhua Li
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  5. Sook Moon
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  6. Mi Ryung Roh
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Correspondence to Mi Ryung Roh or Zhehu Jin.

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Zhenlong Zheng and Lianhua Zhu contributed equally to this study.

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Zheng, Z., Zhu, L., Zhang, X. et al. RUNX3 expression is associated with sensitivity to pheophorbide a-based photodynamic therapy in keloids. Lasers Med Sci 30, 67–75 (2015). https://doi.org/10.1007/s10103-014-1614-4

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  • DOI: https://doi.org/10.1007/s10103-014-1614-4

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