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Phototherapy and resistance training prevent sarcopenia in ovariectomized rats

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Abstract

The aim of this study was to histologically and biochemically analyze the effects of light-emitting diode therapy (LEDT) associated with resistance training to prevent sarcopenia in ovariectomized rats. Forty female Wistar rats (12 months old, 295–330 g) were bilaterally ovariectomized and divided into four groups (n = 10 per group): control–sedentary (C), resistance training (T), LEDT–sedentary (L), and LEDT plus resistance training (LT). Trained rats performed a 12-week water-jumping program (3 days per week) carrying a load equivalent to 50–80 % of their body mass strapped to their back. Depending on the group protocol, the LED device (850 nm, 100 mW, 120 J/cm2, spot size 0.5 cm2) was used either as the only method or after the resistance training had been performed. The device was used in the single point contact mode (for 10 min). The irradiated region was the center of the greater trochanter of the right femur and the middle third of the rectus femoris muscle was subsequently analyzed histomorphometrically. Significant increases (p < 0.05) were noted for the muscle volume of the T (68.1 ± 19.7 %), the L (74.1 ± 5.1 %), and the LT (68.2 ± 11.5 %) groups compared to the C group (60.4 ± 5.5 %). There were also significant increases in the concentrations of IGF-1, IL-1, and TNF-α in the muscles of the treated groups (p < 0.05). Animals in the LT group showed a significant increase in IL-6 compared to T, L, and C groups (p < 0.05). These findings suggest that resistance training and LEDT can prevent sarcopenia in ovariectomized rats.

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Acknowledgments

The authors would like to thank Felippe Furlan, Júlio César Domingues, Luis Vaz de Lima Júnior, and Mariana Sabino Bortolozzo for valuable technical assistance. We would also like to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for their financial support.

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Authors and Affiliations

  1. Department of Morphology, Piracicaba Dental School (FOP), University of Campinas (UNICAMP), Av. Limeira, 901, 13414-903, Piracicaba, SP, Brazil

    Adalberto Vieira Corazza, Francisco Carlos Groppo & Paulo Henrique Ferreira Caria

  2. Optics Group from Physics Institute of São Carlos (IFSC), University of São Paulo (USP), Av. Trabalhador Sãocarlense, 400–Centro, 13560-970, São Carlos, SP, Brazil

    Fernanda Rossi Paolillo & Vanderlei Salvador Bagnato

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  1. Adalberto Vieira Corazza
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Correspondence to Adalberto Vieira Corazza.

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Corazza, A.V., Paolillo, F.R., Groppo, F.C. et al. Phototherapy and resistance training prevent sarcopenia in ovariectomized rats. Lasers Med Sci 28, 1467–1474 (2013). https://doi.org/10.1007/s10103-012-1251-8

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