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The efficacy of the use of IR laser phototherapy associated to biphasic ceramic graft and guided bone regeneration on surgical fractures treated with wire osteosynthesis: a comparative laser fluorescence and Raman spectral study on rabbits

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Abstract

The aim of the present study was to assess, by Raman spectroscopy and laser fluorescence, the repair of surgical fractures fixed with wire osteosynthesis treated or not with infrared laser (λ780 nm, 50 mW, 4 × 4 J/cm2 = 16 J/cm2, ϕ = 0.5 cm2, CW) associated or not to the use of hydroxyapatite and guided bone regeneration. Surgical tibial fractures were created under general anesthesia on 15 rabbits that were divided into five groups, maintained on individual cages, at day/night cycle, fed with solid laboratory pelted diet, and had water ad libitum. The fractures in groups II, III, IV, and V were fixed with wires. Animals in groups III and V were grafted with hydroxyapatite (HA) and guided bone regeneration (GBR) technique used. Animals in groups IV and V were irradiated at every other day during 2 weeks (4 × 4 J/cm2, 16 J/cm2 = 112 J/cm2). Observation time was that of 30 days. After animal death, specimens were taken and kept in liquid nitrogen and used for Raman spectroscopy. The Raman results showed basal readings of 1,234.38 ± 220. Groups WO + B + L showed higher readings (1,680.22 ± 822) and group WO + B the lowest (501.425 ± 328). Fluorescence data showed basal readings of 5.83333 ± 0.7. Groups WO showed higher readings (6.91667 ± 0.9) and group WO + B + L the lowest (1.66667 ± 0.5). There were significant differences between groups on both cases (p < 0.05). Pearson correlation was negative and significant (R 2  = −0.60; p  < 0.001), and it was indicative that, when the Raman peaks of calcium hydroxyapatite (CHA) are increased, the level of fluorescence is reduced. It is concluded that the use of near-infrared lasertherapy associated to HA graft and GBR was effective in improving bone healing on fractured bones as a result of the increasing deposition of CHA measured by Raman spectroscopy and decrease of the organic components as shown by the fluorescence readings.

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Notes

  1. Beam area was measured with a calibrated digital caliper.

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Authors and Affiliations

  1. Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil

    Antônio Luiz Barbosa Pinheiro, Nicole Ribeiro Silva Santos, Priscila Chagas Oliveira, Gilberth Tadeu Santos Aciole, Thais Andrade Ramos, Tayná Assunção Gonzalez, Laís Nogueira da Silva & Artur Felipe Santos Barbosa

  2. Universidade Camilo Castelo Branco Núcleo do Parque Tecnológico de São José dos Campos, Rodovia Presidente Dutra, km. 138 - Distrito de Eugenio de Melo, São José dos Campos, 12247-004, SP, Brazil

    Antônio Luiz Barbosa Pinheiro & Landulfo Silveira Jr

  3. Instituto Nacional de Ciência e Tecnologia de Óptica e Fotônica, São Carlos, SP, Brazil, 13560-970

    Antônio Luiz Barbosa Pinheiro

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  1. Antônio Luiz Barbosa Pinheiro
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  2. Nicole Ribeiro Silva Santos
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Correspondence to Antônio Luiz Barbosa Pinheiro.

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Pinheiro, A.L.B., Santos, N.R.S., Oliveira, P.C. et al. The efficacy of the use of IR laser phototherapy associated to biphasic ceramic graft and guided bone regeneration on surgical fractures treated with wire osteosynthesis: a comparative laser fluorescence and Raman spectral study on rabbits. Lasers Med Sci 28, 815–822 (2013). https://doi.org/10.1007/s10103-012-1166-4

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